Summary
A spontaneous streptomycin-resistant Escherichia coli mutant which is temperature-sensitive for suppression of a nonsense codon was studied for its ability to propagate phages T2, T4D, T5, ϕK, f2, MS2, R17, Qβ, λ as well as filamentous phages fl, fd and M13. Of all phages tested, only the growth of Qβ, λ, and filamentous phages is inhibited in the mutant at 42° C. This selective inhibition suggests that, like Qβ, λ and filamentous phages also require a read-through protein(s) which results from suppression of a termination codon.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barrell, B.G., Air, G.M., Hutchison, C.A.III: Overlapping genes in phage ϕX174. Nature 264, 33–40 (1976)
Belfort, M., Noff, D., Oppenheim, A.B.: Isolation, characterization and deletion mapping of amber mutations in the CII gene of phage λ. Virology 63, 147–159 (1975)
Berkowitz, S.A., Day, L.A.: Mass, length, composition and structure of the filamentous bacterial virus fd. J. Mol. Biol. 102, 531–548 (1976)
Engelberg-Kulka, H., Dekel, L., Israeli-Reches, M.: Streptomycinresistant Escherichia coli mutant temperature-sensitive for the production of Qβ-infective particles. J. Virol. 21, 1–6 (1977)
Engelberg-Kulka, H., Israeli-Reches, M., Dekel, L., Friedmann, A.: Qβ-defective particles produced in a streptomycin-resistant Escherichia coli mutant. J. Virol. In press (1979)
Hirsch, D., Gold, L.: Translation of the UGA triplet in vitro by trytophan transfer RNA's. J. Mol. Biol. 58, 459–468 (1971)
Horiuchi, K., Vovis, G.F., Enea, V., Zinder, N.D.: Cleavage map of phage fl. Location of the E. coli B-specific modification sites. J. Mol. Biol. 95, 147–165 (1975)
Horiuchi, K., Webster, R.E., Matsuhashi, S.: Gene products of bacteriophage Qβ. Virology 45, 429–439 (1971)
Konings, R.N.H., Hulsebos, T., Van den Hondel, C.A.: Identification and characterization of the in vitro synthesized gene products of phage M13. J. Virol. 15, 570–584 (1975)
Kopchick, J.J., Jamjoon, G.A., Watson, K.F., Arlinghouse, R.B.: Biosynthesis of reverse transcriptase from Raucher murine leukemia virus by synthesis and cleavage of a gag-pol readthrough viral precursor polyprotein. Proc. Natl. Acad. Sci. U.S.A. 75, 2016–2020 (1978)
Linney, E., Hayashi, M.: Two proteins of gene A of ϕX174. Nature New Biol. 254, 6–8 (1973)
Lyons, L.B., Zinder, N.D.: The genetic map of the filamentous phage fl. Virology 49, 45–60 (1972)
Marvin, D., Hohn, B.: Filamentous bacterial viruses. Bacteriol. Rev. 33, 179–209 (1966)
Model, P., Zinder, N.D.: In vitro synthesis of phage fl proteins. J. Mol. Biol. 83, 231–251 (1974)
Moore, C.H., Farron, F., Bohnert, D., Weissman, C.: Possible origin of a minor virus-specific protein (A1) in Qβ particles. Nature New Biol. 234, 204–206 (1971)
Pelham, H.R.B.: Leaky UAG termination codon in tobacco mosaic virus RNA. Nature 272, 469–471 (1971)
Philipson, L., Andersson, P., Olshevsky, U., Weinberg, R., Baltimore, D., Gesteland, R.: Translation of murine leukemia and sarcoma virus RNAs in nuclease-treated reticulocyt extracts: Enhancement of gag-pol polypeptide with yeast suppressor tRNA. Cell 13, 189–199 (1978)
Seeburg, P.H., Schaller, H.: Mapping and characterization of promoters in phages fd, fl and M13. J. Mol. Biol. 92, 261–279 (1975)
Shaw, D.C., Walker, J.E., Northrop, F.D., Barrell, B.G., Godson, G.N., Fiddes, J.C.: Gene K, a new overlapping gene in bacteriophage G4. Nature 272, 510–515 (1978)
Taketo, A.: Host genes involved in the replication of single-stranded DNA phage ϕK. Mol. Gen. Genet. 148, 25–29 (1976)
Van den Hondel, C.A., Weijers, A., Konings, R.N.H., Schoenmakers, J.G.G.: Studies on phage M13 DNA. I. A cleavage map of the M13 genome. Eur. J. Biochem. 53, 547–558 (1975)
Vovis, G.F., Horiuchi, K., Zinder, N.D.: Endonuclease R Eco RII restriction of phage fl DNA in vitro: ordering of genes V and VII. location of an RNA promotor for gene VIII. J. Virol. 16, 674–684 (1975)
Weiner, A.M. and Weber, K.: Natural read-through at the UGA termination signal of Qβ coat protein cistron. Nature New Biol. 234, 206–209 (1971)
Yates, J.L., Gette, W.R., Furth, M.E., Nomura, M.: Effects of ribosomal mutations on read-through of a chain termination signal. Studies on the synthesis of bacteriophage λ 0 gene in vitro. Proc. Natl. Acad. Sci. U.S.A. 74, 689–693 (1977)
Zeevi, M., Daniel, V., Engelberg-Kulka, H.: A streptomycin-resistant E. coli mutant with ribosomes temperature-sensitive in the suppression of a nonsense codon. Mol. Gen. Genet. 170, 149–153 (1979)
Author information
Authors and Affiliations
Additional information
Communicate by H. G. Wittmann
Rights and permissions
About this article
Cite this article
Engelberg-Kulka, H., Dekel, L., Israeli-Reches, M. et al. The requirement of nonsense suppression for the development of several phages. Molec. gen. Genet. 170, 155–159 (1979). https://doi.org/10.1007/BF00337791
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00337791