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Primary structure and promoter analysis of leghemoglobin genes of the stem-nodulated tropical legume Sesbania rostrata: Conserved coding sequences, cis-elements and trans-acting factors

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Summary

The primary structure of a leghemoglobin (lb) gene from the stem-nodulated, tropical legume Sesbania ostrata and two lb gene promoter regions was analysed. The S. rostrata lb gene structure and Lb amino acid composition were found to be highly conserved with previously described lb genes and Lb proteins. Distinct DNA elements were identified in the S. rostrata lb promoter regions, which share a high degree of homology with cis-active regulatory elements found in the soybean (Glycine max) lbc3 promoter. One conserved DNA element was found to interact specifically with an apparently universal, trans-acting factor present in nuclear extracts of nodules. These results suggest a conserved mechanism for nodule specific induction of lb genes in leguminous plants.

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Authors and Affiliations

  1. Abteilung Genetische Grundlagen der Pflanzenzüchtung, Max-Planck-Institut für Züchtungsforschung, D-5000, Köln 30, Federal Republic of Germany

    Birgit A. Metz, Peter Welters, Hans Jürgen Hoffman, Jeff Schell & Frans J. de Bruijn

  2. Department of Molecular Biology and Plant Physiology, University of Aarhus, DK-8000, Aarhus, Denmark

    Erik Ø. Jensen

Authors
  1. Birgit A. Metz
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  2. Peter Welters
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  3. Hans Jürgen Hoffman
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  4. Erik Ø. Jensen
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  5. Jeff Schell
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  6. Frans J. de Bruijn
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Communicated by H. Saedler

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Metz, B.A., Welters, P., Hoffman, H.J. et al. Primary structure and promoter analysis of leghemoglobin genes of the stem-nodulated tropical legume Sesbania rostrata: Conserved coding sequences, cis-elements and trans-acting factors. Mol Gen Genet 214, 181–191 (1988). https://doi.org/10.1007/BF00337709

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  • DOI: https://doi.org/10.1007/BF00337709

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