Summary
Pedunculate oak seedlings (Quercus robur) inoculated with the ectomycorrhizal fungus Laccaria lacata were grown for 1 year on fertilized sphagnum peat in two nurseries. Three factors affecting microbial populations in the substrate were studied, fungicide treatment of the seeds, peat disinfection before sowing (methyl bromide or steam pasteurization), and inoculation with mycorrhization helper bacteria. Treatment of acorns with Iprodione had no depressive effect on mycorrhiza formation. Both disinfection techniques were equivalent, stimulating or depressing mycorrhiza formation depending on the initial microflora in the peat. The introduction of two previously selected mycorrhization helper bacteria (one Pseudomonas fluorescens and one unidentified fluorescent pseudomonad), isolated from L. laccata sporocarps associated with Douglas fir—L. laccata ectomycorrhizas in other nurseries, significantly increased the mycorrhizal rate from 30 to 53% of the short roots. The implications of these results for the controlled mycorrhization of planting stocks and the specificity of mycorrhization helper bacteria are discussed.
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References
Delatour C (1978) Recherche d'une méthode de lutte curative contre le Ciboria batschiana chez les glands. Eur J For Pathol 8:193–200
Delran S, Garbay J, Le Tacon F (1975) Production rapide de plants feuillus sur torube fertilisée: Nouveaux résultats. Rev For Française 27:437–448
Delran S, Garbaye J, Kabré A, Le Tracon F, Perrin R (1982) La production rapide de plants feuillus sur tourbe fertilisé: Les problèmes microbiologiques posés par la réutilisation de la tourbe pendant plusieurs années consécutives. Rev For Française 34:314–325
De Oliveira VL, Garbaye J (1989) Les microorganismes auxiliaines de l'établissement des symbioses ectomycorhiziennes. Eur J For Path 19:54–64
Duponnois R, Garbaye J (1990) Some mechanisms involved in growth stimulation of ectomycorrhizal fungi by bacteria. Can J Bot 68:2148–2152
Duponnois R, Garbaye J (1991a) Mycorrhization helper bacteria associated with the Douglas fir—Laccaria laccata symbiosis: Effects in aseptic and in glasshouse conditions. Ann Sci For 48:239–251
Duponnois R, Garbaye J (1991b) Effect of dual inoculation of Douglas fir with the extomycorrhizal fungus Laccaria laccata and mycorrhization helper bacteria (MHB) in two bare-root forest nurseries. Plant and Soil 47:370–375
Garbaye J (1991) Biological interactions in the rhizosphere. Experientia 47:370–375
Garbaye J, Bowen GD (1987) Effect of different microflora on the success of ectomycorrhizal inoculation of Pinus radiata. Can J For Res 17:941–943
Garbaye J, Bowen GD (1989) Stimulation of ectomycorrhizal infection of Pinus radiata by some microorganisms associated with the mantle of ectomycorrhizas. New Phytol 112:383–388
Le Tacon F (1974) Recherche des meilleures conditions de production des plants de hêtre. Rev For Française 26:299–305
Pachlewski R, Pachlewska J (1974) Studies on symbiotic properties of mycorrhizal fungi of pine (Pinus sylvestris L.) with the aid of the method of mycorrhizal synthesis in pure culture on agar. Forest Research Institute, Warsaw, p 228
Rovira AD, Bowen GD (1966) The effects of microorganisms upon plant growth: II. Detoxication of heat sterilized soils by fungi and bacteria. Plant and Soil 25:129–142
Trappe JM, Molina R, Castellano M (1984) Reactions of mycorrhizal fungi and mycorrhiza formation to pesticides. Annu Rev Phytopathol 22:331–359
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Garbaye, J., Churin, JL. & Duponnois, R. Effects of substrate sterilization, fungicide treatment, and mycorrhization helper bacteria on ectomycorrhizal formation of pedunculate oak (Quercus robur) inoculated with Laccaria laccata in two peat bare-root nurseries. Biol Fert Soils 13, 55–57 (1992). https://doi.org/10.1007/BF00337239
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DOI: https://doi.org/10.1007/BF00337239