Summary
Electron microscopical studies dealt with the reversible action of Ro4-1284 on norepinephrine and serotonin with respect to the granulated vesicles of the ventromedial hypothalamus and the pineal body of the rat. In the normal rat various types of vesicles were classified according to their size and form. In the hypothalamus two types of granulated vesicles were distinguished from the ungranulated ones. In the pineal gland granular and agranular vesicles have the same diameter; in addition to them large granular vesicles were also to be found.
After the application of Ro4-1284 the same types of vesicles appear, which are almost totally deprived of their osmiophilic content. Homogeneous vesicles partially containing membranous bodies remain. The results were interpreted as a visible depletion of norepinephrine and possibly serotonin from their pools which leave behind elements capable of becoming functionally active after the action of the depleting agent.
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Literature
Bak, I.: Electron microscopic observations in the substantia nigra of mouse during reserpine administration. Experientia (Basel) 21, 568–570 (1965).
Bertler, A., B. Falck, and Ch. Owman: Cellular localization of 5-HT in the rat pineal gland. Kungl. Fysiogr. Sällsk. i. Lund Förh. 33, 13–16 (1961).
Bondareff, W.: Submicroscopic morphology of granular vesicles in sympathetic nerves of rat pineal body. Z. Zellforsch. 67, 211–218 (1965).
Clementi, F.: Modifications ultrastructurelles provoquées par quelques médicaments sur les terminaisons nerveuses adrénergiques et sur la médullaire surrénale. Experientia (Basel) 21, 171–176 (1965).
Hökfelt, T.: The effect of reserpine on the intraneuronal vesicles of the rat vas deferens. Experientia (Basel) 22, 56 (1966).
Ishii, S., N. Shimizu, M. Matsuoka, and R. Imaizumi: Correlation between catecholamine content and numbers of granulated vesicles in rabbit hypothalamus. Biochem. Pharmacol. 14, 183–184 (1965).
Matsuoka, M., S. Ishii, N. Shimizu, and R. Imaizumi: Effect of Win 18501-2 on the content of catecholamines and the number of catecholamine-containing granules in the rabbit hypothalamus. Experientia (Basel) 21, 121–123 (1965).
Owman, Ch.: Sympathetic nerves probably storing two types of monoamines in the rat pineal gland. Int. J. Neuropharm. 2, 105–112 (1964).
Pellegrino de Iraldi, A., and E. de Robertis: Action of reserpine, iproniazid and pyrogallol on nerve endings of the pineal gland. Int. J. Neuropharmacol. 2, 231–239 (1963).
—, H. Duggan, and E. de Robertis: Adrenergic synaptic vesicles in the anterior hypothalamus of the rat. Anat. Rec. 145, 4, 521–531 (1963a).
Pletscher, A., A. Brossi, and K. Gey: Benzoquinolizine derivatives: a new class of monoamine decreasing drugs with psychotropic action. Int. Rev. Neurobiol. 4, 275–306 (1962).
Quay, W. B., and A. Halevy: Experimental modification of the rat pineal's content of serotonin and related indole amines. Physiol. Zool. 1, 1–7 (1962).
Reynolds, E. S.: The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Sano, Y., u. T. Mashimo: Elektronenmikroskopische Untersuchungen an der Epiphysis cerebri beim Hund. Z. Zellforsch. 69, 129–139 (1966).
Sulser, F., and F. Sovoko: On the role of rate of brain norepinephrine release in the antibenzoquinolizine action of desimipramine. Psychopharmacol. 8, 191–200 (1965).
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Halaris, A., Rüther, E. & Matussek, N. Effect of a benzoquinolizine (RO 4-1284) on granulated vesicles of the rat brain. Z. Zellforsch. 76, 100–107 (1967). https://doi.org/10.1007/BF00337034
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DOI: https://doi.org/10.1007/BF00337034