Summary
We have analyzed 11 strains and clones, representing five species (Penicillium janthinellum, P. citrioviridae, P. chrysogenum, Aspergillus niger, Trichoderma harzianum) and three genera of filamentous fungi, for the presence of hypervariable loci in their genomes by hybridization with simple repeat oligonucleotides and the DNA of phage M13. The oligonucleotide probes (CT)8, (GTG)5 and (GACA)4, as well as M13 DNA, are informative probes for fingerprinting in all genera and species tested. The probe (GATA)4 produced informative fingerprints only with the genomic DNA of A. niger. There was no similarity between the fingerprints originating from fungi of different genera and also little similarity between the fingerprints of different species belonging to the same genus. Fingerprints of strains of the same species differed only slightly from each other. Fingerprints of clones originating from one strain were identical. The results indicate that DNA fingerprinting is a powerful method to differentiate species and strains of filamentous fungi.
This is a preview of subscription content, log in via an institution to check access.
References
Barnett JA (1983) Yeast: characteristics and identification. Cambridge University Press, Cambridge, UK
Beyermann B, Nürnberg P, Weihe A, Meixner M, Epplen J, Börner T (1991) Theor Appl Genet (in press)
Braithwaite KS, Manners JM (1989) Curr Genet 16:473–475
Epplen JT (1988) J Hered 79:409–417
Georges M, Lequarré AS, Castelli M, Hanset R, Vassart G (1988) Cytogenet Cell Genet 47:127–131
Heeswijck R van, Roncero MIG (1984) Carlsberg Res Commun 46:691–702
Huey B, Hall J (1989) J Bacteriol 175:2528–2532
Jeffreys AJ, Wilson V, Thein SL (1985) Nature 314:67–73
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Rogstad SH, Patton JC, Schaal BA (1988) Proc Natl Acad Sci USA 85:9176–9178
Ryshkov AP, Jincharadse AG, Prosniak MI, Ivanov PL, Limborska SA (1988) FEBS Lett 233:388–392
Schäfer R, Zischler H, Birsner U, Becker A, Epplen JT (1988) Electrophoresis 9:369–374
Schöld M, Colombero A, Reyes AA (1984) DNA 3:469–477
Tautz D (1989) Nucleic Acids Res 17:6463–6471
Uitterlinden AG, Slagboom PE, Knook DL, Vijk J (1989) Proc Natl Acad Sci USA 86:2742–2746
Vassart G, Georges M, Monsieur R, Brocas H, Lequarré AS, Christophe D (1987) Science 235:683–684
Walmsley RM, Wikinson BM, Kong TH (1989) Bio/Technol 7:1168–1170
Weide H, Aurich H (1979) Allgemeine Mikrobiologie. Gustav Fischer Verlag, Jena
Weihe A, Niemann C, Lieckfeld D, Meyer W, Börner T (1990) Fingerprint News 2:9–10
Weising K, Weigand F, Driesel AJ, Kahl G, Zischler H, Epplen JT (1989) Nucleic Acids Res 17:10128
Wöstemeyer J (1985) Eur J Biochem 146:443–448
Author information
Authors and Affiliations
Additional information
Communicated by K. Esser
Rights and permissions
About this article
Cite this article
Meyer, W., Koch, A., Niemann, C. et al. Differentiation of species and strains among filamentous fungi by DNA fingerprinting. Curr Genet 19, 239–242 (1991). https://doi.org/10.1007/BF00336493
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00336493