Summary
The complete genomic sequence of the inducible Chlorella kessleri H+/hexose cotransporter (HUP1) has been obtained from two overlapping clones isolated from a λgt10 library. The HUP1 gene is interrupted by 14 introns with the first intron being located in the 5′-untranslated part of the gene. The average intron length is 220 bp, yielding a very regular intron/exon pattern in the gene. The codon usage in this gene is strongly biased with a clear preference for C and a strong suppression of A. A consensus sequence for a putative algal polyadenylation sequence is shown and compared with other algal cDNA sequences.
Similar content being viewed by others
References
Bendich AJ, Anderson RS, Ward BL (1979) In Leaver CJ (ed) Genome organization and expression in plants. Plenum Press, New York, pp
Brown JWS (1986) Nucleic Acids Res 14:9549–9559
Chiang K-S, Sueoka N (1967) Proc Natl Acad Sci USA 57:1506–1513
Cho B-H, Sauer N, Komor E, Tanner W (1981) Proc Natl Acad Sci USA 78:3591–3594
Ertl H, Mengele R, Wenzl S, Engel J, Sumper M (1989) J Cell Biol 109:3493–3501
Goldschmidt-Clermont M, Rahire M (1986) J Mol Biol 191:421–432
Hanahan D (1983) J Mol Biol 166:557–558
Hirano H, Takeda Y, Iwamura T (1986) Plant Mol Biol 7:311–317
Hostos EL de, Schilling J, Grossman AR (1989) Mol Gen Genet 218:229–239
Houlne G, Schantz R (1987) Curr Genet 12:611–612
Komor E (1973) FEBS Lett 38:16–18
Komor E, Tanner W (1974) J Gen Physiol 64:568–581
Maniatis T, Fritsch EF, Sambrook J (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Mayfield SP, Rahire M, Frank G, Zuber H, Rochaix J-D (1987) Proc Natl Acad Sci USA 84:749–753
Merchant S, Bogorad L (1987) J Biol Chem 262:9062–9067
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Sauer N, Tanner W (1984) Z Pflanzenphysiol 114:367–375
Sauer N, Tanner W (1989) FEBS lett 259-43-46
Sauer N, Caspari T, Klebl F, Tanner W (1990) Proc Natl Acad Sci USA 87 (in press)
Silflow CD, Chrisholm RL, Conner TW, Ranum LP (1985) Mol Cell Biol 5:2389–2398
Tanner W (1969) Biochem Biophys Res Commun 36:278–283
Youngblom J, Schloss JA, Silflow CD (1984) Mol Cell Biol 4:2686–2696
Yu LM, Selman BR (1988) J Biol Chem 263:19342–19345
Author information
Authors and Affiliations
Additional information
Communicated by H. Kössel
Rights and permissions
About this article
Cite this article
Wolf, K., Tanner, W. & Sauer, N. The Chlorella H+/hexose cotransporter gene. Curr Genet 19, 215–219 (1991). https://doi.org/10.1007/BF00336489
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00336489