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Prolonged simulated acid rain treatment in the subarctic: Effect on the soil respiration rate and microbial biomass

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Abstract

Humus chemistry and respiration rate, ATP, ergosterol, and muramic acid concentration as measures of chemical properties, microbial activity, biomass, and indicators of fungal and bacterial biomass were studied in a long-term acid rain experiment in the far north of Finnish Lapland. The treatments used in this study were dry control, irrigated control (spring water, pH 6), and two levels of simulated acid rain (pH 4 and pH 3). Originally (1985–1988), simulated acid rain was prepared by adding both H2SO4 and HNO3 (1.9:1 by weight). In 1989 the treatments were modified as follows. In subarea 1 the treatments continued unchanged (H2SO4+HNO3 in rain to pH 4 and pH 3), but in subarea 2 only H2SO4 was applied. The plots were sampled in 1992. The acid application affected humus chemistry by lowering the pH, cation exchange capacity, and base saturation (due to a decrease in Ca and Mg) in the treatment with H2SO4+HNO3 to pH 4 (total proton load over 8 years 2.92 kmol ha-1), whereas the microbial variables were not affected at this proton load, and only the respiration rate decreased by 20% in the strongest simulated acid rain treatment (total proton load 14.9 kmol ha-1). The different ratios of H2SO4+HNO3 in subareas 1 and 2 did not affect the results.

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References

  • Alexander M (1980) Effects of acidity on micro-organisms and microbial processes in soil. In: Hutchinson TC, Havas M (eds) Effects of acid precipitation on terrestrial ecosystems. Plenum Press, New York, pp 363–374

    Google Scholar 

  • Bååth E, Lundgren B, Söderström B (1979) Effects of artificial acid rain on microbial activity and biomass. Bull Environ Contam Toxicol 23: 737–740

    Google Scholar 

  • Bäck J, Neuvonen S, Huttunen S (1994) Pine needle growth and fine structure after prolonged acid rain treatment in the subarctic. Plant Cell Environ 17:1009–1021

    Google Scholar 

  • Bewley RJF, Stotzky G (1983) Anionic constituents of acid rain and microbial activity in soil. Soil Biol Biochem 15:431–437

    Google Scholar 

  • Bitton G, Boylan RA (1985) Effects of acid precipitation on soil microbial activity: I. Soil core studies. J Environ Qual 14:66–69

    Google Scholar 

  • Bringmark E, Bringmark L (1991) Large-scale pattern of mor layer degradability in Sweden measured as standardized respiration. Lect Notes Earth Sci 33:255–259

    Google Scholar 

  • Brown KA (1985) Acid deposition: Effect of sulphuric acid at pH 3 on chemical and biochemical properties of bracken litter. Soil Biol Biochem 17:31–38

    Google Scholar 

  • Falkengren-Grerup U (1987) Long-term changes in the pH of forest soils in southern Sweden. Environ Pollut 43:79–90

    Google Scholar 

  • Fritze H (1992) Effects of environmental pollution on forest soil microflora-a review. Silva Fenn 26:37–47

    Google Scholar 

  • Fritze H, Smolander A, Levula T, Kitunen V, Mälkönen E (1994) Wood-ash fertilization and fire treatments in a Scots pine forest stand: Effects on the organic layer, microbial biomass, and microbial activity. Biol Fertil Soils 17:57–63

    Google Scholar 

  • Grant WD, West AW (1986) Measurement of ergosterol, diaminopimelic acid and glucosamine in soil: Evaluation as indicators of microbial biomass. J Microbiol Methods 6:47–53

    Google Scholar 

  • Guggenberger G, Glaser B, Zech W (1994) Heavy metal binding by hydrophobic and hydrophilic dissolved organic carbon fractions in a spodosol A and B horizon. Water Air Soil Pollut 72:111–127

    Google Scholar 

  • Hämet-Ahti L (1963) Zonation of the mountain birch forest in northernmost Fennoscandia. Annales botanici Societatis zoologicae-botanicae Fennicae 34, 4:1–127

    Google Scholar 

  • Järvinen O, Vänni T (1990) Bulk deposition chemistry in Finland. In: Kauppi B, Anttila P, Kenttämies K (eds) Acidification in Finland. Springer-Verlag, Berlin Heidelberg, pp 151–165

    Google Scholar 

  • Kallio P, Laine U, Mäkinen Y (1969) Vascular flora of Inari Lapland. I. Introduction and Lycopodiaceae — Polypodiaceae. Rep Kevo Res Sta 5:1–108

    Google Scholar 

  • Kauppi P, Anttila P, Kenttämies K (1990) Acidification in Finland. Springer-Verlag, Berlin Heidelberg

    Google Scholar 

  • Kauppi PE, Mielikäinen K, Kuusela K (1992) Biomass and carbon budget of European forests, 1971 to 1990. Science 256:70–74

    Google Scholar 

  • Killham K, Firestone MK, McColl JG (1983) Acid rain and soil microbial activity: Effects and their mechanisms. J Environ Qual 12:133–137

    Google Scholar 

  • Kuusela K, Mattila E, Salminen S (1986) Forest resources in north Finland by forestry board districts, 1982 to 1984. Folia For 655:1–86

    Google Scholar 

  • Kytöviita M-M, Fritze H, Neuvonen S (1990) The effects of acidic irrigation on soil microorganisms at Kevo. Northern Finland. Environ Pollut 66:21–31

    Google Scholar 

  • von Lützow M, Zelles L, Scheunert I, Ottow JCG (1992) Seasonal effects of liming, irrigation, and acid precipitation on microbial biomass N in a spruce (Picea abies L.) forest soil. Biol Fertil Soils 13:130–134

    Google Scholar 

  • McColl JG, Firestone MK (1991) Soil chemical and microbial effects of simulated acid rain on clover and soft chess. Water Air Soil Pollut 60:301–313

    Google Scholar 

  • Moore TR (1987) The effect of simulated acid rain on the nutrient status of subarctic woodland soil in eastern Canada. Can J For Res 17:370–378

    Google Scholar 

  • Moriarty DJW (1983) Measurement of muramic acid in marine sediments by high performance liquid chromatography. J Microbiol Methods 1:111–117

    Google Scholar 

  • Nohrstedt H-Ö (1985) Biological activity in soil from forest stands in central Sweden, as related to site properties. Microb Ecol 11:259–266

    Google Scholar 

  • Nohrstedt H-Ö (1987) A field study on forest floor respiration response to artificial heavy metal contaminated acid rain. Scand J For Res 2:13–19

    Google Scholar 

  • Neuvonen S, Suomela J (1990) The effect of simulated acid rain on pine needle and birch leaf litter decomposition. J Appl Ecol 27:857–872

    Google Scholar 

  • Neuvonen S, Suomela J, Haukioja E, Lindgren M, Ruohomäki K (1990) Ecological effects of simulated acid rain in a subarctic area with low ambient sulphur deposition. In: Kauppi P, Anttila P, Kenttämies K (eds) Acidification in Finland, Springer Verlag, Berlin Heidelberg, pp 477–493

    Google Scholar 

  • Nygaard PH, Abrahamsen G (1991) Effects of long-term artificial acidification on the ground vegetation and soil in a 100 year-old stand of Scots pine (Pinus sylvestris) Plant and Soil 131:151–160

    Google Scholar 

  • Persson T, Lundkvist H, Wiren A, Hyvönen R, Wessen B (1989) Effects of acidification and liming on carbon and nitrogen mineralization and soil organism in mor humus. Water Air Soil Pollut 45:77–96

    Google Scholar 

  • Rönkkö R, Pennanen T, Smolander A, Kitunen V, Kortemaa H, Haahtela K (1994) Quantification of Frankia strains and other root-associated bacteria in pure cultures and in the rhizosphere of axenic seedlings by high-performance liquid chromatography-based muramic acid assay Appl Environ Microbiol 60:3672–3678

    Google Scholar 

  • Salonius PO (1990) Respiration rates in forest soil organic horizon materials treated with simulated acid rain. Can J For Res 20:910–913

    Google Scholar 

  • Sokal R, Rohlf FJ (1981) Biometry, 2nd edn. Freeman, New York

    Google Scholar 

  • Tuovinen J-P, Laurila T, Lättilä H, Ryaboshapko A, Brukhanov P, Korovlev S (1993) Impact of the sulphur dioxide sources in the Kola peninsula on air quality in northernmost Europe. Atmos Environ 27A:1379–1395

    Google Scholar 

  • Trolldenier G (1989) Plant nutritional and soil factors in relation to microbial activity in the rhizosphere, with particular emphasis on denitrification. Z Pflanzenernähr Bodenkd 152:223–230

    Google Scholar 

  • Vanhala P, Ahtiainen J (1994) Soil respiration, ATP content and Photobacterium toxicity test as indicators of metal pollution in soil. Environ Toxicol Water Qual 9:115–121

    Google Scholar 

  • Vanhala P, Kiikkilä O, Fritze H (1995) Microbial responses of forest soil to moderate anthropogenic air pollution: A large scale field survey. Water Air Soil Pollut (in press)

  • de Vries W, Breeuwsma A (1987) The relation between soil acidification and element cycling. Water Air Soil Pollut 35:293–310

    Google Scholar 

  • Wanner M, Funke I, Funke W (1994) Effects of liming, fertilization and acidification on pH, soil moisture, and ATP concentration of soil from a spruce forest in southern Germany. Biol Fertil Soils 17:297–300

    Google Scholar 

  • Will ME, Graetz DA, Roof BS (1986) Effect of simulated acid precipitation on soil microbial activity in a Typic Quartzipsamment. J Environ Qual 15:399–403

    Google Scholar 

  • Wolters V (1991a) Effects of acid rain on leaf-litter decomposition in a beech forest on calcareous soil. Biol Fertil Soils 11:151–156

    Google Scholar 

  • Wolters V (1991b) Biological processes in two beech forest soils treated with simulated acid rain — a laboratory experiment with Isotoma Tigrina (insecta, collenbola). Soil Biol Biochem 23:381–390

    Google Scholar 

  • Zelles L (1988) The simultaneous determination of muramic acid and glucosamine in soil by high-performance liquid chromatography with precolumn fluorescence derivatisation. Biol Fertil Soils 6:125–130

    Google Scholar 

  • Zelles L, Scheunert I, Kreutzer K (1987) Effect of artificial irrigation, acid precipitation and liming on the microbial activity in soil of a spruce forest. Biol Fertil Soils 4:137–143

    Google Scholar 

  • Zelles L, Stepper K, Zsolnay A (1990) The effect of lime on microbial activity in spruce (Picea abis L.) forests. Biol Fertil Soils 9:87–82

    Google Scholar 

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Authors and Affiliations

  1. Finnish Environment Agency, P.O. Box 140, FIN-00251, Helsinki, Finland

    P. Vanhala

  2. Finnish Forest Research Institute, P.O. Box 18, FIN-01301, Vantaa, Finland

    H. Fritze

  3. Subarctic Research Institute, University of Turku, FIN-20500, Turku 50, Finland

    S. Neuvonen

Authors
  1. P. Vanhala
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  2. H. Fritze
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  3. S. Neuvonen
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Vanhala, P., Fritze, H. & Neuvonen, S. Prolonged simulated acid rain treatment in the subarctic: Effect on the soil respiration rate and microbial biomass. Biol Fertil Soils 23, 7–14 (1996). https://doi.org/10.1007/BF00335811

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  • DOI: https://doi.org/10.1007/BF00335811

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