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Oxidative enzymes, glycogen and lipid in striated muscle

A histochemical study in the atlantic hagfish (Myxine glutinosa (L.))

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Summary

  1. 1.

    By means of current methods oxidative enzymes, glycogen and lipid have been localized on the histological and cytological level in Myxine. musculature.

  2. 2.

    Red muscle fibres (belonging to the “slow” physiological variety) and heart muscle cells contain large quantities of most enzymes studied as well as of glycogen. In addition red fibres of m. parietalis contain large quantities of storage lipid.

  3. 3.

    White fibres (which are typical “fast” fibres) contain practically no cytochrome oxidase and Krebs' cycle dehydrogenases, except for some activity of NAD-dependent malic dehydrogenase. However, these fibres are approximately as rich in lactate dehydrogenase and NAD-dependent α-glycerophosphate dehydrogenase as red fibres. Glycogen content of different fibres varies somewhat, mostly it stains equal in red, white and intermediate fibres.

  4. 4.

    Intermediate fibres generally show an intermediate activity of enzymes and content of glycogen and lipid.

    Non-NAD-dependent α-glycerophosphate, glucose-6-phosphate, and β—hydroxybutyrate dehydrogenases were not detected by the histochemical methods used.

  5. 5.

    These observations are discussed in relation to physiological and ultrastructural findings in Myxine as well as to investigations on muscle of other animals.

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Abbreviations

SDH:

Succinate dehydrogenase

α-GPDH (M):

“Menadione-linked” α-glycerophosphate dehydrogenase

α-GPDH (NAD):

NAD-linked α-glycerophosphate dehydrogenase

β-HO-BDH:

β-hydroxybutyrate dehydrogenase

CYO:

Cytochrome oxydase

G-6-PDH:

Glucose-6-phosphate dehydrogenase

IDH (NAD):

NAD-linked isocitrate dehydrogenase

IDH (NADP):

NADP-linked isocitrate dehydrogenase

LDH:

Lactate dehydrogenase

MDH (NAD):

NAD-linked malate dehydrogenase

MDH (NADP):

NADP-linked malate dehydrogenase

NBT:

Nitro blue tetrazolium

PMS:

Phenazine methosulfate

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Authors and Affiliations

  1. Anatomical Institute, University of Oslo, Norway

    Svein Ivar Mellgren & Jon Storm Mathisen

Authors
  1. Svein Ivar Mellgren
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  2. Jon Storm Mathisen
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Additional information

This study was supported by The Norwegian Research Council for Science and the Humanities and by U. S. Public Health Service (Grant NB 02215-06).

The authors are indebted to Professor Dr. philos. Bjørn Føyn and Cand. real. Finn Walvig, Biological Station, University of Oslo. Drøbak, for generous supply of hagfish material. We also wish to thank Professor Jan Jansen, M. D. and Associate Professor Th. Blackstad, M. D. for reading the manuscript, and Mrs. A. Holter and Mr. E. Risnes for technical assistance.

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Mellgren, S.I., Mathisen, J.S. Oxidative enzymes, glycogen and lipid in striated muscle. Zeitschrift für Zellforschung 71, 169–188 (1966). https://doi.org/10.1007/BF00335745

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