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Yeast ARS function and nuclear matrix association coincide in a short sequence from the human HPRT locus

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Summary

A sequence that supports extrachromosomal replication of plasmids in yeast has been identified within the first intron of the human hypoxanthine-guanine phosphori bosyltransferase (HPRT) gene. This represents the first isolation of such an autonomously replicating sequence (ARS) from an exactly known position in the human genome. This ARS shares similarities of imparted yeast phenotype and DNA sequence with other heterologus ARSs. In addition, this sequence is found to be a matrix association region (MAR) on the basis of specific binding to nuclear matrices prepared from several mammalian cell types. It also exhibits anomalous electrophoretic behavior, characteristic of bent DNA, on polyacrylamide gels. The coincidence of these properties supports the possibility that this region may play a role in DNA replication within its normal chromosomal context.

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Authors and Affiliations

  1. Department of Biochemistry, Baylor College of Medicine, 77030, Houston, TX, USA

    Robert C. Sykes, Dong Lin & A. Craig Chinault

  2. Institute for Molecular Genetics, Baylor College of Medicine, 77030, Houston, TX, USA

    Sung J. Hwang, Paul E. Framson & A. Craig Chinault

Authors
  1. Robert C. Sykes
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  2. Dong Lin
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  3. Sung J. Hwang
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  4. Paul E. Framson
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  5. A. Craig Chinault
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Communicated by R. Devoret

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Sykes, R.C., Lin, D., Hwang, S.J. et al. Yeast ARS function and nuclear matrix association coincide in a short sequence from the human HPRT locus. Mol Gen Genet 212, 301–309 (1988). https://doi.org/10.1007/BF00334700

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  • DOI: https://doi.org/10.1007/BF00334700

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