Summary
Three tandemly arranged repeats (A, B, C) of 16S and 23S rDNA, and one supplementary (S) 16S rDNA adjacent to the 16S rDNA of repeat A, are present within an 18 kbp segment of a circular 73 kbp DNA from the colourless flagellate Astasia longa. The repeat units are separated by a short region containing a 5S rRNA gene and a gene for tRNA-Val (UAC). Sequence comparisons reveal 78%, 81%, and 67% identical nucleotides of the 23S rDNA (A), the 16S rDNA (B), and the 5S rDNA (A), respectively, with the corresponding genes of the Euglena gracilis chloroplast genome. As in Euglena chloroplasts, the 3′-terminal protion of the 23S rDNA is homologous to the 4.5S rRNA gene of higher plant chloroplast genomes. These results are supportive of a common evolutionary origin for the Astasia 73 kbp DNA and the Euglena 145 kbp chloroplast DNA.
Similar content being viewed by others
References
Blum JJ, Sommer JR, Kahn V (1965) J Protozool 12:202–209
Brosius J, Dull TJ, Noller HF (1980) Biochem 77:201–204
Brosius J, Dull TJ, Sleeter DD, Noller HF (1981) J Mol Biol 148:107–127
Chen EY, Seeburg PH (1985) DNA 4: 165
Christopher DA, Hallick RB (1989) Nucleic Acids Res 17: 7591–7608
Edwards K, Kössel H (1981) Nucleic Acids Res 9:2853–2869
Gingrich JC, Hallick RB (1985) J Biol Chem 260:16156–16161
Graf L, Kössel H, Stutz E (1980) Nature 286:908–910
Graf L, Roux E, Stutz E (1982) Nucleioc Acids Res 10: 6369–6381
Gutell RR, Fox GE (1988) Nucleic Acids Res 16:r175-r269
Hallick RB, Buetow DE (1989) In: Buetow DE (ed) The biology of Euglena, vol IV. Academic Press, San Diego, pp 351–414
Karabin GD, Narita JO, Dodd JR, Hallick RB (1983) J Biol Chem 258:14790–14796
Kivic PA, Vesk M (1973) Can J Bot 52:695–699
Kössel H, Natt E, Strittmatter G, Fritzsche E, Gozdzicka-Jozefiak A, Przybyl D (1985) In: Vloten-Doting L van, Groot GSP, Hall TC (eds) Molecular form and function of the plant genome. Plenum Press, New York, pp 183–198
Koller B, Roux E, Montandon PE, Stutz E (1988) Plant Mol Biol 10:339–348
Maly P, Brimacombe R (1983) Nucleic Acids Res 11:7263–7286
Maniatis T, Fritzsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Montandon PE, Stutz E (1983) Nucleic Acids Res 11:5877–5892
Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322:572–574
Orozco EM, Rushlow KE, Dodd JR, Hallick RB (1980) J Biol Chem 255:10997–11003
Orozco EM, Hallick RB (1982) J Biol Chem 257:3265–3275
Palmer JD (1985) Annu Rev Genet 19:325–354
Rochaix JD, Darlix JL (1982) J Mol Biol 159:383
Ruf M, Kössel H (1988) FEBS Lett 240:41–44
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Shine J, Dalgarno L (1974) Proc Natl Acad Sci USA 71:1342–1346
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamagashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Siemeister G (1989) Dissertation, Bonn
Siemeister G, Buchholz C, Hachtel W (1990) Mol Gen Genet 220:425–432
Siemeister G, Hachtel W (1989) Curr Genet 15:435–441
Siemeister G, Hachtel W (1990) Plant Mol Biol (in press)
Steege DA, Graves MC, Spremulli LL (1982) J Biol Chem 257:10430–10439
Takaiwa F, Sugiura M (1982) Eur J Biochem 124:13–19
Wolters J, Erdmann VA (1988) Nucleic Acids Res 16:r1-r70
Yamada T, Shimaji M (1987) Curr Genet 11:347–352
Yepiz-Plascencia GM, Jenkins ME, Hallick RB (1988) Nucleic Acids Res 16:9340
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Siemeister, G., Hachtel, W. Organization and nucleotide sequence of ribosomal RNA genes on a circular 73 kbp DNA from the colourless flagellate Astasia longa . Curr Genet 17, 433–438 (1990). https://doi.org/10.1007/BF00334524
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00334524