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Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance

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Summary

Low frequency, integrative transformation of three fertile hermaphroditic strains of Magnaporthe grisea has been achieved using plasmid pAN7-1 and cosmid pAN7-2, which contain an Escherichia coli hygromycin B phosphotransferase gene linked to Aspergillus nidulans regulatory sequences.

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References

  • Amasino RM (1986) Anal Biochem 152:304–307

    Google Scholar 

  • Crawford MF, Chumley FG, Weaver CG, Valent B (1986) Genetics 114:1111–1129

    Google Scholar 

  • Daboussi MJ, Djeballi A, Gerlinger C, Blaiseau PL, Bouvier I, Cassan M, Lebrun MH, Parisot D, Brygoo Y (1989) Curr Genet 15:453–456

    Google Scholar 

  • Ellingboe AH, Wu BC, Robertson W (1990) Phytopathology (in press)

  • Kato H, Yamaguchi T (1982) Ann Phytopathol Soc Jpn 48:607–612

    Google Scholar 

  • Leong SA, Skinner D, Leung H, Karjalainen R, Ellingboe A (1989) Fungal Genetics Newsletter 36:12

    Google Scholar 

  • Leung H, Taga M (1988) Adv Plant Pathol 6:175–188

    Google Scholar 

  • Leung H, Williams PH (1985) Can J Genet Cytol 27:697–704

    Google Scholar 

  • Leung H, Borromeo ES, Bernardo MA, Notteghem JL (1988) Phytopathology 78:1227–1233

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Manicom BQ, Bar-Joseph M, Rosner A, Vigodsk-Haas H, Kotze JM (1987) Phytopathology 77:669–672

    Google Scholar 

  • Parsons KA, Chumley FG, Valent B (1987) Proc Natl Acad Sci USA 84:4161–4165

    Google Scholar 

  • Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CAMJJ (1987) Gene 56:117–124

    Google Scholar 

  • Turgeon G, Garber RC,Yoder OC (1987) Mol Cell Biol 7:3297–3305

    Google Scholar 

  • Valent B, Crawford MS, Weaver CG, Chumley FG (1986) Iowa State J Res 60:569–594

    Google Scholar 

  • Wang J, Holden DW, Leong SA (1988) Proc Natl Acad Sci USA 85:865–869

    Google Scholar 

  • Yaegashi H (1978) Ann Phytopathol Soc Jpn 44:626–632

    Google Scholar 

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Author notes

  1. Hei Leung

    Present address: Department of Plant Pathology, Washington State University, 99164, Pullman, WA, USA

  2. Ulla Lehtinen & Reijo Karjalainen

    Present address: Department of General Microbiology, University of Helsinki, Mannerheimintie 172, SF-00300, Helsinki, Finland

Authors and Affiliations

  1. Department of Plant Pathology, University of Wisconsin, 53706, Madison, WI, USA

    Hei Leung, Ulla Lehtinen, Reijo Karjalainen, Daniel Skinner, Sally Leong & Albert Ellingboe

  2. USDA Foreign Disease-Weed Science Research Unit, 21701, Frederick, MD, USA

    Paul Tooley

  3. USDA Plant Disease Resistance Research Unit, University of Wisconsin, 53706, Madison, WI, USA

    Sally Leong

Authors
  1. Hei Leung
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  2. Ulla Lehtinen
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  3. Reijo Karjalainen
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  4. Daniel Skinner
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  5. Paul Tooley
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  6. Sally Leong
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  7. Albert Ellingboe
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Leung, H., Lehtinen, U., Karjalainen, R. et al. Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance. Curr Genet 17, 409–411 (1990). https://doi.org/10.1007/BF00334519

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  • DOI: https://doi.org/10.1007/BF00334519

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