Summary
It is shown by the mixed reconstitution technique that in E. coli AJ7 rif-rJ7 rifampicin resistance resides in the β-subunit. Thus, the otherwise isogenic pair of strains AJ1 rif-s and AJ7 rif-rJ7 are suitable as a reference for genetic work on transcription. In addition, comparison of cyanogen bromide fragments of both strains by 2-dimensional electrophoresis exhibits a difference in one fragment probably due to charge density and not size, and therefore presumably an amino-acid exchange.
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Communicated by H. G. Wittmann
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Boyd, D.H., Zillig, W. & Scaife, F.J.G. Reference mutations for the β subunit of RNA polymerase. Molec. gen. Genet. 130, 315–320 (1974). https://doi.org/10.1007/BF00333870
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DOI: https://doi.org/10.1007/BF00333870