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Mutagenic DNA repair in Escherichia coli

VIII. Involvement of DNA polymerase III in constitutive and Inducible mutagenic repair after ultraviolet and gamma irradiation

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Summary

Mutagenic repair in Escherichia coli after ultraviolet (UV) irradiation has previously been shown to require a function of DNA polymerase III. In contrast, no effect of incubating a polC temperature-sensitive strain at 42° has been found after gamma irradiation. Thus at present there is no direct evidence for the involvement of polymerase III in gamma ray mutagenesis. This could, however, merely reflect the stability of the premutational lesion during the period of polymerase III insufficiency such that mutagenic repair is resumed on the plate during subsequent incubation at permissive temperature.

It was previously suggested that an inducible factor might interact with polymerase III to enable it to polymerise in an error-prone way in daughter strand gaps opposite non-coding lesions in the template strand. A temperature-resistant revertant (CM 792) of a temperature-sensitive polC strain (CM 731) has been isolated which has properties expected of a strain in which the polymerase III complex is no longer susceptible to the inducible co-factor. Its UV sensitivity, spontaneous mutation rate and mutagenic response to ethyl methanesulphonate are all normal or near normal, also the rates of mutation to prototrophy after gamma irradiation and to streptomycin resistance after UV. These latter mutations are believed to arise through constitutive mutagenic repair at sites in pre-existing DNA. In contrast, the rate of UV-induced mutation to prototrophy due to changes at ochre suppressor loci is greatly depressed and no Weigle-reactivation of bacteriophage T3 is observable; both these effects are believed to result from the action of inducible mutagenic repair in newly-replicated DNA. It is suggested that the 3′ to 5′ exnnuclease activity of the polymerase III complex in CM 792 may not be susceptible to inhibition by an inducible factor and so continues to remove “mismatched” bases inserted in newly-replicated DNA opposite damage template sites thus preventing the fixation of errors as mutations.

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Authors and Affiliations

  1. M.R.C. Cell Mutation Unit, University of Sussex, Falmer, BN1 9QG, Brighton, England

    B. A. Bridges & R. P. Mottershead

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  1. B. A. Bridges
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  2. R. P. Mottershead
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Communicated by H. Böhme

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Bridges, B.A., Mottershead, R.P. Mutagenic DNA repair in Escherichia coli . Molec. Gen. Genet. 162, 35–41 (1978). https://doi.org/10.1007/BF00333848

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  • DOI: https://doi.org/10.1007/BF00333848

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