Summary
It is shown that mutants of Saccharomyces cerevisiae able to efficiently utilise exogenous dTMP can also utilise exogenous dAMP. Under extracellular conditions permissive for dTMP uptake label stemming from offered [8-3H]dAMP is incorporated preferentially into alkali-resistant, high molecular weight material (putative DNA): only about 30% of high molecular weight cell-bound dAMP label was found to be sensitive towards mild alkali hydrolysis. This putative RNA label can be minimised to practically zero when ≧mM Ade is employed in a dAMP labelling assay. Exogenous dAMP at ≫10 μM was found to be cytostatic similarly to ≫μM dTMP and similarly to inhibit effectively import of exogenous Pi. We conclude from our results that there exists a yeast cytoplasmic membrane permease able to import dAMP. A model of this hypothetical permease system is presented.
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Abbreviations
- S. cerevisiae :
-
Saccharomyces cerevisiae
- TIP :
-
yeast cytoplasmic membrane permease importing dTMP under permisive conditions
- AIP :
-
hypothetical yeast cytoplasmic membrane permease importing dAMP under permissive conditions
- tlr :
-
symbol for the recessive genetic trait to utilise effeciently exogenous dTMP
- tmp :
-
symbol for the recessive genetic trait leading to dTMP auxotrophy
- dTMP:
-
2′-deoxythymidine 5′-monophosphate
- dAMP:
-
2′-deoxyadenosine 5′-monophosphate
- (d)NMP:
-
(deoxy)ribonucleoside 5′-monophosphate
- dThd:
-
2′-deoxythymidine
- Thy:
-
thymine
- dAdo:
-
2′-deoxyadenosine
- Ado:
-
adenosine
- Ade:
-
adenine
- Ura:
-
uracil
- Pi :
-
inorganic phosphate
References
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Communicated by F. Kaudewitz
Apart from discrete abbreviations we followed the rules of nomenclature as recommended by the IUPAC-IUB commission of biochemical nomenclature (CBN)
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Fäth, W.W., Brendel, M. Nucleic acid metabolism in yeast. Molec Gen Genet 188, 121–127 (1982). https://doi.org/10.1007/BF00333005
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DOI: https://doi.org/10.1007/BF00333005