Summary
It is shown that highly efficient utilisers of exogenous dTMP of the yeast Saccharomyces cerevisiae are able to excrete the nucleotide with similar efficiency. Strains Pi-repressible in acid phosphatase/nucleotidase excrete dTMP at extracellular high Pi; strains constitutive for this enzymic activity excrete dThd. Excretion of thymidylate and dThd, unlike uptake of exogenous dTMP, seems to be unaffected by the extracellular pH, by the extracellular presence of dTMP, and to be rather independent of the extracellular presence of a metabolisable carbohydrate such as D(+)-glucose. A model of the yeast dTMP-incorporation principle (TIP) is presented suggesting that it is also responsible for export of endogenous thymidylate.
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Abbreviations
- S. cerevisiae :
-
Saccharomyces cerevisiae
- dTMP:
-
2′-deoxythymidine 5′-monophosphate
- dThd:
-
2′-deoxythymidine
- Thy:
-
thymine
- Pi :
-
inorganic phosphate
- TIP :
-
S. cerevisiae cytoplasmic membrane permease importing dTMP
- tlr :
-
symbol to designate a recessive mutated state of the yeast genome locus TLR which results in a more or less enhanced TIP activity
- (tlr):
-
phenotypic symbol for tlr accounting for loss of α mating type of the tlr mutants used
References
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Communicated by F. Kaudewitz
Apart from discrete abbreviations we followed the rules of nomenclature recommended by the IUPAC-IUB commission of biochemical nomenclature (CBN)
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Fäth, W.W., Brendel, M. Nucleic acid metabolism in yeast. Molec Gen Genet 188, 115–120 (1982). https://doi.org/10.1007/BF00333004
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DOI: https://doi.org/10.1007/BF00333004