Summary
Spermatogonia of adult men contain frequently the so-called crystalloid of Lubarsch. This rod-shaped structure consists of a bundle of fine tubules running parallel to the long axis. The tubules (100 Å) are connected with one another. This structure is also observed in the spermatocyte occasionally and is similar to the crystalloid of Charcot-Böttcher in the cytoplasm of human Sertoli cells. In chick spermatogonia, a homologous crystalloid occurs infrequently. Morphological similarities of the crystalloids to those occurring in other cells are discussed. Mitochondria in the human spermatogonium show some aggregation; between them dense intermitochondrial material was noted.
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References
André, J.: Contribution à la connaissance du chondriome. J. Ultrastruct. Res., Suppl. 3 (1962).
Bawa, S. R.: Fine structure of the Sertoli cell of the human testis. J. Ultrastruct. Res. 9, 459–474 (1963).
Bennett, H. S., and J. H. Luft: s-Collidine as a basis for buffering fixatives. J. biophys. biochem. Cytol. 6, 113–114 (1959).
Bensch, K. G., and S. E. Malawista: Microtubular crystals in mammalian cells. J. Cell Biol. 40, 95–107 (1969).
Bulger, R. E., Granule-lamella complex in monkey renal proximal tubular cells. J. Ultrastruct. Res. 24, 150–156 (1968).
Byers, B.: Ribosome crystallization induced in chick embryo tissues by hypothermia. J. Cell Biol. 30, C1-C6 (1966).
Clermont, Y.: The cycle of seminiferous epithelium of man. Amer. J. Anat. 112, 35–51 (1963).
—: Renewal of spermatogonia in man. Amer. J. Anat. 118, 509–524 (1966).
Farbman, A. I.: A particle-membrane complex in developing rat taste buds. J. Ultrastruct. Res. 19, 514–521 (1967).
Fawcett, D. W., and D. M. Phillips: Further observations on mammalian spermatogenesis. J. Cell Biol. 35, 152A (1967).
Gatenby, J. B., and H. W. Beams: The cytoplasmic inclusions in the spermatogenesis of man. Quart. J. micr. Sci. 78, 1–29 (1935).
Ito, T.: Über den Golgiapparat und die Mitochondrien der Spermatogonien sowie Spermatozyten des Menschen, nebst Bemerkungen der Riesenspermatogonien. Cytologia (Tokyo) 11, 436–451 (1941).
Kessel, R. G.: Annulate lamellae. J. Ultrastruct. Res., Suppl. 10 (1968).
Kretser, D. M. de: The fine structure of the immature human testis in hypogonadotrophic hypogonadism. Virchows Arch. Abt. B Zellpath. 1, 283–296 (1968).
Lubarsch, O.: Über das Vorkommen krystallinischer und krystalloider Bildungen in den Zellen des menschlichen Hodens. Virchows Arch. path. Anat. 145, 316–338 (1896).
Luft, J. H.: The use of acrolein as a fixative for light and electron microscopy. Anat. Rec. 133, 305 (1959).
—: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Morgan, R. S., and B. G. Uzman: Nature of the packing of ribosomes within chromatoid body. Science 152, 214–216 (1968).
Nagano, T.: Some observations on the fine structure of the Sertoli cell in the human testis. Z. Zellforsch. 73, 89–106 (1966).
—: Fine structure of cells and tissues III, 67. Tokyo: Igaku Shoin (1967).
Odor, D. L.: The ultrastructure of unilaminar follicles of the hamster ovary. Amer. J. Anat. 116, 493–522 (1965).
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Sabatini, D. D., K. Bensch, and R. J. Barrnett: Cytochemistry and electron microscopy: the preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963).
Sohval, A. R.: Personal communication (1968).
Stieve, H.: Männliche Geschlechtsorgane. In: Handbuch der mikroskopischen Anatomie des Menschen, herausgeg. von W. v. Möllendorff, Bd. 7/2. Berlin: Springer 1930.
Swift, H.: The fine structure of annulate lamellae. J. biophys. biochem. Cytol. 2, No 4 Suppl. 415–418 (1956).
Szollosi, D.: (1968) (Quoted from Bulger, 1968).
Tres, L. L., and A. J. Solari: The ultrastructure of the nuclei and the behaviour of the sex chromosomes of human spermatogonia. Z. Zellforsch. 91, 75–89 (1968).
Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475–478 (1958).
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Study supported by grants (HD 00593-05) from U.S.P.H.S. and the Japanese Ministry of Education. The author wishes to thank members of Urology Department, Chiba University for supplying biopsy materials and Dr. G. Yasuzumi for assistance in the bibliography.
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Nagano, T. The crystalloid of Lubarsch in the human spermatogonium. Z. Zellforsch. 97, 491–501 (1969). https://doi.org/10.1007/BF00332798
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DOI: https://doi.org/10.1007/BF00332798