Summary
We report here the complete nucleotide sequence of the E. coli triose phosphate isomerase gene. The gene encodes a polypeptide of 255 amino acids which is approximately 46% homologous to eukaryotic triose phosphate isomerases, and approximately 38% homologous to the enzyme from a thermophilic bacterium, Bacillus stearothermophilus. The nucleotide sequence is 55% homologous to that of the corresponding gene in the yeast Saccharomyces cerevisiae. To our knowledge, this is the first report of the sequence of a gene coding a glycolytic enzyme from a prokaryotic organism.
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References
Alber T, Banner DW, Bloomer AC, Petsko GA, Phillips DC, Rivers PS, Wilson IA (1981) On the three-dimensional structure and catalytic mechanism of triose phosphate isomerase. Philos Trans R Soc Lond (Biol) 293:159–171
Alber T, Kawasaki G (1982) Nucleotide sequence of the triose phosphate isomerase gene of Saccharomyces cerevisiae. J Mol App Genet 1:419–434
Artavanis-Tsakonas S, Harris JI (1980) Primary structure of triose phosphate isomerase from Bacillus Stearothermophilus. Eur J Biochem 108:599–611
Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev March 1980, pp 1–56
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Res 7:1513–1523
Clarke L, Carbon J (1976) A colony bank containing synthetic ColE1 hybrid plasmids representative of the entire E. coli genome. Cell 9:91–99
Corran PH, Waley SG (1975) The amino acid sequence of the rabbit muscle triose phosphate isomerase. Biochem J 145:335–344
Drouin J (1980) Cloning of human mitochondrial DNA in Escherichia coli. J Mol Biol 140:15–34
Fahey RC, Kolb E, Harris JI (1971) Triose phosphate isomerase from Bacillus stearothermophilus. Biochem J 124: 77P
Fraenkel DG, Vinopal RT (1973) Carbohydrate metabolism in bacteria. Annu Rev Microbiol 27:69–100
Furth AJ, Milman JD, Priddle JD, Offord RE (1974) Studies on the subunit structure and amino acid sequence of triose phosphate isomerase from chicken breast muscle. Biochem J 139:11–25
Gold L, Pribnow D, Schneider T, Shinedling S, Singer BS, Stormo G (1981) Translational initiation in procaryotes. Annu Rev Microbiol 35:365–403
Grosjean H, Fiers W (1982) Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes. Gene 18:199–209
Kolb E, Harris JI, Bridgen J (1974) Triose phosphate isomerase from coelocanth. Biochem J 137:185–197
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 227:680–685
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, N. Y.
Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavage. Methods Enzymol 65:499–560
Pichersky E, Gottlieb LD (1983) Evidence for duplications of the structural genes coding plastid and cytosolic isozymes of triose phosphate isomerase in diploid species of Clarkia. Genetics 105:421–436
Pichersky E, Gottlieb LD, Higgins RC (1984) Hybridization between subunits of triose phosphate isomerase isozymes from different subcellular compartments of higher plants. Mol Gen Genet 193:158–161
Pichersky E, Gottlieb LD (1984) Plant triose phosphate isomerase isozymes: Purification, immunological and structural characterization, and partial amino acid sequences. Plant Physiol 74:340–347
Rodriguez RL, Tait RC (1983) Recombinant DNA Techniques: An Introduction. Addison-Wesley Publishing Company Inc. Reading, Massachusetts
Rosenber M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–353
Shimosaka M, Fukuda Y, Murata K, Kimura A (1982) Application of hybrid plasmids carrying glycolytic genes to ATP production by Escherichia coli. J Bacteriol 152:98–103
Thompson J, Gerstenberger PD, Goldberg DE, Gociar E, Silva AO, Fraenkel DG (1979) ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt. J Bacteriol 137:502–506
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Pichersky, E., Gottlieb, L.D. & Hess, J.F. Nucleotide sequence of the triose phosphate isomerase gene of Escherichia coli . Mol Gen Genet 195, 314–320 (1984). https://doi.org/10.1007/BF00332765
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DOI: https://doi.org/10.1007/BF00332765