Summary
The nucleotide sequence of the gene for the 32,000 dalton thylakoid membrane protein from Nicotiana tabacum has been determined. This gene is located 535 base pairs (bp) away from the inverted repeat on the right margin of the large single-copy region of the chloroplast DNA. The coding region contains 1059 bp (353 codons). The deduced amino acid sequence is identical with those of spinach and Nicotiana debneyi. Transcription of this gene starts 85 bp upstream from the ATG codon and terminates 91–95 bp downstream from the TAA stop codon. A strong homology was observed in the promoter sequences between the genes for the 32,000 dalton protein and the large subunit of ribulose-1,5-bisphosphate carboxylase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bedbrook JR, Link G, Coen DM, Bogorad L, Rich A (1978) Maize plastid gene expressed during photoregulated development. Proc Natl Acad Sci USA 75:3060–3064
Deno H, Shinozaki K, Sugiura M (1983) Nucleotide sequence of tobacco chloroplast gene for the α subunit of proton-translocating ATPase. Nucl Acids Res 11:2185–2191
Driesel AJ, Speirs J, Bohnert HJ (1980) Spinach chloroplast mRNA for a 32,000 dalton polypeptide. Size and localization on the physical map of the chloroplast DNA. Biochim Biophys Acta 610:297–310
Ellis RJ (1981) Chloroplast proteins: Synthesis, transport, and assembly. Ann Rev Plant Physiol 32:111–137
Shinozaki K, and Sugiura M (1982) The nucleotide sequence of the tobacco chloroplast gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Gene 20:91–102
Shinozaki K, Deno H, Kato A, Sugiura M (1983) Overlap and cotranscription of the gene for the beta and epsilon subunits of tobacco chloroplast ATPase. Gene 24:147–155
Spielmann A, Stutz E (1983) Nucleotide sequence of soybean chloroplast DNA region which contain the psb A and trn H genes and cover the ends of the large single copy region and one end of the inverted repeat. Nucl Acids Res 11:7157–7167
Sugita M, Sugiura M (1983) A putative gene of tobacco chloroplast coding for ribosomal protein similar to E. coli ribosomal protein S19. Nucl Acids Res 11:1913–1918
Sugita M, Kato A, Shimada H, Sugiura M (1984) Sequence analysis of the junctions between a large inverted repeat and singlecopy regions in tobacco chloroplast DNA. Mol Gen Genet (in press)
Sugiura M (1980) Purification of the T4 DNA ligase by Blue Sepharose chromatography. Anal Biochim 108:227–229
Tohdoh N, Sugiura M (1982) The complete nucleotide sequence of a 16S ribosomal RNA gene from tobacco chloroplasts. Gene 17:213–218
Zurawski G, Perrot B, Bottomley W, Whitfeld P (1981) The structure of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from spinach chloroplast DNA. Nucl Acids Res 9:3251–3270
Zurawski G, Bohnert HJ, Whitfeld PR, Bottomley W (1982) Nucleotide sequence of the gene for the Mr 32,000 thylakoid membrane protein from Spinacia oleracea and Nicotiana debneyi predicts a totally conserved primary translation product of Mr 38,950. Proc Natl Acad Sci USA 79:7699–7703
Author information
Authors and Affiliations
Additional information
Communicated by M. Takanami
Rights and permissions
About this article
Cite this article
Sugita, M., Sugiura, M. Nucleotide sequence and transcription of the gene for the 32,000 dalton thylakoid membrane protein from Nicotiana tabacum . Mol Gen Genet 195, 308–313 (1984). https://doi.org/10.1007/BF00332764
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00332764