Summary
Evidence is presented showing that the relaxed phenotype of the SY 15 mutant is determined by one nuclear recessive mutation. The most characteristic patterns of the relaxed phenotype in yeast — rRNA accumulation and rRNA processing in the absence of protein synthesis — were found to segregate together in first and second generation crosses. Therefore, the interuption of rRNA processing that occurs after starvation for a required amino acid is a pleiotropic manifestation of the stringent control itself. It is suggested that the locus for the stringent response in Saccharomyces cerevisiae (designated STR) coordinates the synthesis of rRNA on transcriptional and post-transcriptional levels.
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Kelker HC, Pogo AO (1980) The stringent and relaxed phenomena in Saccharomyces cerevisiae. J Biol Chem 255:1526–1535
Kozhina T, Stateva L, Venkov P (1979) Genetic analysis of an osmotic sensitive Saccharomyces cerevisiae mutant. Molec Gen Genet 170:351–354
Nakeda D, Anderson IA, Magasanik B (1964) Fate of ribosomal RNA produced by a relaxed mutant of E. coli. J Mol Biol 9:472–488
Oliver, EW, and McLaughlin, CS (1977) The regulation of RNA synthesis in yeast. I. Starvation experiments. Molec Gen Genet 154:145–153
Shulman RW, Sripati CE, Warner JR (1977) Noncoordinated transcription in the absence of protein synthesis in yeast. J Biol Chem 252:1344–1349
Venkov PV (1979) Low ribonuclease activity in cellular lysates of osmotic sensitive Saccharomyces cerevisiae mutants. Molec Gen Genet 175:1111–112
Venkov PV, Hadjiolov AA, Battaner E, Schlessinger D (1974) Saccharomyces cerevisiae sorbitol dependent fragile mutants. Biochim Biophys Res Commun 56:599–604
Waltschewa L, Georgiev O, Venkov P (1983) Relaxed mutant of Saccharomyces cerevisiae: Proper maturation of ribosomal RNA in absence of protein synthesis. Cell 33:221–230
Warner JR (1982) The yeast ribosome, In: Strathern JN, Jones EW (eds) The Molecular Biology of the Yeast Saccharomyces cerevisiae. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 529–560
Zakharov IA, Kozhin SA, Kozhina TN, Fedorova IV (1976) Methods in genetics of Saccharomyces. Nauka, Leningrad
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Communicated by F. Kaudewitz
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Stateva, L.I., Venkov, P.V. Genetic analysis of Saccharomyces cerevisiae SY 15 relaxed mutant. Mol Gen Genet 195, 234–237 (1984). https://doi.org/10.1007/BF00332752
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DOI: https://doi.org/10.1007/BF00332752