Summary
The gene coding for the 33,000 dalton protein (B protein) of the mini-F plasmid has been mapped by minicell polypeptide analysis to 47.3–48.2 Fkb. Transcription of the gene is initiated near 47.3 Fkb. Gene B mutants overproduce the 42,000 dalton protein (A protein) of mini-F. Insertions of Tn 5 in the B gene and in the incD region cause instability of plasmid inheritance. Mini-F plasmids delected for part of gene B are not maintained in the absence of the incD region. B protein and the incD region appear to interact in promoting mini-F maintenance.
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References
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Communicated by D. Sherratt
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Phua, S.H., Bergquist, P.L. & Lane, H.E.D. Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis. Mol Gen Genet 188, 353–355 (1982). https://doi.org/10.1007/BF00332700
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DOI: https://doi.org/10.1007/BF00332700