Summary
Cloning of mini-F DNA segments has led to the identification and mapping of a locus, incD, involved in incompatibility reactions with many IncFI plasmids. The cloned incD locus expressed incompatibility with F, R386, and six other IncFI plasmids but not with ColV3-K30 or pHH507 which lack sequence homology with the incD region. A sequence of 360 bp (48.66–49.02 FKB) was found to be sufficient for expression of incD incompatibility. Multicopy vectors containing incD are compatible with each other, but can be displaced by mini-F plasmids deleted for incD. These results indicate that incD-mediated incompatibility reactions require the presence of replication genes to which incD is normally linked. The degree of incompatibility exercised by incD is moderate compared with that of other inc loci in F, suggesting that incD is involved in an aspect of plasmid maintennce, such as partition, different from the functions of the other inc loci.
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Bergquist PL, Adelberg EA (1972) Abnormal excision and transfer of chromosomal segments by a strain of Escherichia coli K12. J Bacteriol 111:119–128
Bergquist PL, Jamieson AF, Gardner RC, Lane D (1980) Replication mutants of the F-plasmid of Escherichia coli K12. I. Isolation and characterization of temperature-sensitive replication mutants of F′-gal +. Plasmid 3:165–178
Bergquist PL, Downard RA, Caughey PA, Gardner RC, Lane HED (1981) Analysis of mini-F plasmid replication by transposition mutagenesis. J Bacteriol 147:888–899
Bergquist PL, Lane HED, Malcolm L, Downard RA (1982) Molecular homology and incompatibility in the IncFI plasmid group. J Gen Microbiol 128
Bolivar F, Rodriguez RL, Betlach MC, Boyer HW (1977) Construction and characterization of new cloning vehicles. I. Ampicillin resistant derivatives of the plasmid pMB9. Gene 2:75–93
Chang ACY, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol 134:1141–1156
Collins J, Brüning HJ (1978) Plasmids usable as gene-cloning vectors in an in vitro packaging by coliphage λ: ‘cosmids’. Gene 4:85–107
Dennison S (1972) Naturally occurring R factor, derepressed for pilus synthesis belonging to the same incompatibility group as the sex factor F of Escherichia coli K12. J Bacteriol 109:416–422
Gardner RC (1979) ‘Replication functions of the F plasmid’. PhD Thesis, University of Auckland
Gardner RC, Caughey PA, Lane HED, Bergquist PL (1980) Replication mutants of the F-plasmid of Escherichia coli. II. Cloned replication regions of temperature sensitive mutants. Plasmid 3:179–192
Kahn ML, Figurski D, Ito L, Helinski DR (1979) Essential regions for replication of a stringent and a relaxed plasmid in Eschrichia coli. Cold Spring Harbor Symp Quant Biol 43:99–103
Kline BC (1979) Incompatibility between F′lac, R386, and F:pSC101 recombinant plasmids: the specificity of F incompatibility genes. Plasmid 2:437–445
Kline BC, Lane HED (1980) A proposed system for nomenclature for incompatibility genes of the Escherichia coli sex factor, plasmid F. Plasmid 4:231–232
Kline BC, Palchaudhuri S (1980) Genetic studies of F plasmid maintenance genes. Plasmid 4:281–291
Lane HED, Chandler M (1977) Mapping of the drug resistance genes carried by the r-determinant of the R100-1 plasmid. Mol Gen Genet 157:17–23
Lane HED, Gardner RC (1979) Second EcoRI fragment of F capable of self-replication. J Bacteriol 139:141–151
Lovett MA, Helinski DR (1976) Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F Km plasmid. J Bacteriol 127:982–989
Manis JJ, Kline BC (1978) F plasmid incompatibility and copy number genes: their map location and interactions. Plasmid 1:492–507
Meacock PA, Cohen SN (1980) Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance. Cell 20:529–542
Palchaudhuri S, Maas WK (1977) Physical mapping of a DNA sequence common to plasmids of incompatibility group FI. Proc Natl Acad Sci USA 74:1190–1194
Phua S-H, Bergquist PL, Lane HED (1982) Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis. Mol Gen Genet 188:353–355
Pinney RJ, Jacob AE, Hedges RW, Smith JT (1977) Dislodgement and thymineless elimination of N-group plasmids. J Gen Microbiol 30:21–30
Taylor DP, Cohen SN (1979) Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy mutant of R1. J Bacteriol 137:92–104
Timmis KN, Andres I, Slocombe PM (1978) Plasmid incompatibility: cloning analysis of an incFII determinant of R6-5. Nature 273:27–32
Tolun A, Helinski DR (1981) Direct repeats of the F plasmid incC region express F incompatibility. Cell 24:687–694
Wehlmann H, Eichenlaub R (1980) Plasmid mini-F encoded proteins. Mol Gen Genet 180:205–211
Wehlmann H, Eichenlaub R (1981) Analysis of transcripts from plasmid mini-F by electron microscopy of R-loops. Plasmid 5:259–266
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Gardner, R.C., Malcolm, L., Bergquist, P.L. et al. IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group. Mol Gen Genet 188, 345–352 (1982). https://doi.org/10.1007/BF00332699
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DOI: https://doi.org/10.1007/BF00332699