Summary
In previously irradiated endosperm cells of Haemanthus katherinae studied in vitro by means of micro-cinematography, two-kinetochore chromatids and dicentric chromosomes have been observed. Breaking of such dicentric chromatids and chromosomes has been analysed. Behaviour of some of the dicentric chromosomes during anaphase deserves special attention: interlocking dicentrics cut one through another and rejoin in a few minutes. In this way from a metaphase interlocking dicentric, two sister anaphase dicentrics are formed. Interlocked dicentrics can also uncoil and not break at all. In this case no activity was observed in one kinetochore of one dicentric in later stages of anaphase (two kinetochores were active in one dicentric and only one in its sister). Analysis of chromosome movements in two-kinetochore chromatids and dicentrics is also presented.
Similar content being viewed by others
References
Bajer, A.: Cine-micrographic studies on chromosome movements in β-irradiated cells. Chromosoma (Berl.) 9, 319–331 (1958).
- and J. Molè-Bajer: Mitosis in endosperm. III. B. 16 mm film (1962a). - Cine analysis of some aspects of mitosis in endosperm. In Cinematography in cell Biology, ed. G. G. Rose. New York: Academic Press 1963 (b).
Cornman, I.: A summary of evidence in favour of the traction fibre in mitosis. Amer. Naturalist 78, 410–422 (1944).
Darlington, C. D., and A. P. Wylie: A dicentric cycle in Narcissus. Heredity 6, Suppl., 197–213 (1953).
Giles, N. H.: Radiation — induced chromosome aberrations in Tradescantia. In: Radiation biology vol. 1, p. 713–761 ed. A. Hollaender. New York: McGraw Hill Book Co. 1954.
Hair, J. B.: The origin of new chromosomes in Agropyron. Heredity 6, Suppl., 215–233 (1953).
Kaufmann, B. P.: Chromosome aberrations induced in animal cells by ionizing radiations. In: Radiation biology, ed. A. Hollaender, vol. 1, p. 627–711, New York: McGraw Hill Book Co. 1954.
Koller, P. C.: Dicentric chromosomes in a rat tumor induced by an aromatic nitrogen mustard. Heredity 6, Suppl., 181–196 (1953).
McClintock, B.: The stability of broken ends of chromosomes in Zea Mays. Genetics 26, 234–282 (1941).- The fusion of broken ends of chromosomes following nuclear fusion. Proc. nat. Acad. Sci. (Wash.) 28, 458–463 (1942).
Molé-Bajer, J.: (1963 — in preparation).
- and A. Bajer: Mitosis in endosperm. Technique of studies. La Cellule (1963 in the press).
Östergren, G., and A. Bajer: (1963 — in preparation).
—, J. Molè-Bajer and A. Bajer: An interpretation of transport phenomena at mitosis. Ann. N. Y. Acad. Sci. 90, 381–408 (1960).
Swanson, C. P.: Cytology and cytogenetics. London: Mcmillan Co. 1958.
Wolff, S.: Radiation genetics. In: Mechanisms in radiobiology, ed. by M. Errera and A. Forssberg. New York: Academic Press 1961.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bajer, A. Observations on dicentrics in living cells. Chromosoma 14, 18–30 (1963). https://doi.org/10.1007/BF00332608
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00332608