Summary
Non-saponifiable cell extracts of wild type and sterol mutants of N. crassa were analysed by means of gas-liquid chromatography. The wild-type contained ergosterol and episterol in a 10:1 ratio. None of the mutants was able to synthesize ergosterol. Three of the mutants carry single recessive gene mutations causing blocks in the terminal steps of ergosterol biosynthesis: erg-1 has an inactive Δ 8→Δ 7 isomerase, erg-2 has an inactive 24(28) hydrogenase, and erg-4 has an inactive C-24 methyl transferase. Some of the mutants accumulated novel sterols as a result of their enzyme defects. The genes erg-1 and erg-2 were mapped close to inl on the right arm of chromosome V.
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Communicated by F. Kaudewitz
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Grindle, M., Farrow, R. Sterol content and enzyme defects of nystatin-resistant mutants of Neurospora crassa . Molec. Gen. Genet. 165, 305–308 (1978). https://doi.org/10.1007/BF00332531
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DOI: https://doi.org/10.1007/BF00332531