Summary
A grpE mutation of Escherichia coli K12, which blocks DNA replication of the phage λ (Saito and Uchida, 1977), was mapped at 56 min on the standard genetic map. A transducing λ phage, λgrpE22, carrying the wild type allele of the grpE gene was constructed in vitro. Structures of λgrpE22 and its viable deletion derivatives were determined by electron microscopic analyses of appropriate heteroduplexes. Proteins coded by the bacterial DNA incorporated into the transducing phages were detected by two-dimensional gel electrophoresis. The results showed that the product of the grpE gene is a weakly acidic protein of molecular weight 24,000. Structural genes for two ribosomal proteins, rplS (L19) and rpsP (S16) were also shown to be carried by λgrpE22.
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References
Adler, J., Kaiser, A.D.: Mapping of the galactose genes of Escherichia coli by transduction with phage P1. Virology 19, 117–126 (1963)
Apirion, S., Watson, N.: Mapping and characterization of a mutation in Escherichia coli that reduces the level of ribonuclease III specific for double-stranded ribonucleic acid. J. Bact. 124, 317–324 (1975)
Appleyard, R.K.: Segregation of lambda lysogenicity during bacterial recombination in E. coli K12. Genetics 39, 429–439 (1954)
Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bact. Rev. 40, 116–167 (1976)
Böck, A., Ruffler, D., Piepersberg, W., Wittmann, H.G.: Genetic analysis of an alteration of ribosomal protein S20 in revertants of an alanyl-tRNA-synthetase mutant of Escherichia coli. Molec. gen. Genet. 134, 325–332 (1974)
Cameron, J.R., Panasenko, S.M., Lehman, I.R., Davis, R.W.: In vitro construction of bacteriophage λ carrying segments of the Escherichia coli chromosome: Selection of hybrids containing the gene for DNA ligase. Proc. nat. Acad. Sci. (Wash.) 72, 3426–3420 (1975)
Davidson, N., Szybalski, W.: Physical and chemical characteristics of lambda DNA. In: The bacteriophage lambda (A.D. Hershey (ed.), pp. 45–82. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1971
Davis, R.W., Simon, M., Davidson, N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Methods in enzymology (S.P. Colowick and N.O. Kaplan, eds.), Vol. 21D, pp. 413–428. New York: Academic Press 1971
De Lucia, P., Cairns, J.: Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature (Lond.) 224, 1164–1166 (1969)
Enquist, L.W., Weisberg, R.A.: The red plaque test: A rapid method for identification for excision defective variants of bacteriophage lambda. Virology 72, 147–153 (1976)
Georgopoulos, C.P., Herskowitz, I.: Escherichia coli mutants blocked in lambda DNA synthesis. In: The bacteriophage lambda (A.D. Hershey, ed.), pp. 553–564. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1971
Hendrix, R.W., Tsui, L.: Role of the host in virus assembly: Cloning of the Escherichia coli groE gene and identification of its protein product. Proc. nat. Acad. Sci. (Wash.) 75, 136–139 (1978)
Ikeuchi, T., Yura, T., Yamagishi, H.: Genetic and physical studies of lambda transducing bacteriophage carrying the beta subunit gene of the Escherichia coli ribonucleic acid polymerase. J. Bact. 122, 1247–1256 (1975)
Jaskunas, S.R., Burgess, R.R., Lindahl, L., Nomura, M.: Identification of a gene for the subunit of RNA polymerase at the str-spc region of the Escherichia coli chromosome. Proc. nat. Acad. Sci. (Wash.) 72, 5036–5040 (1975)
Jaskunas, S.R., Fallon, A.M., Nomura, M.: Identification and organization of ribosomal protein genes of Escherichia coli carried by λfus2 transducing phage. J. biol. Chem. 252, 7323–7336 (1977)
Kaltschmidt, E., Wittmann, H.G.: Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Analyt. Biochem. 36, 401–412 (1970)
Kitakawa, M., Isono, K.: Localization of the structural gene for ribosomal protein L19 (rplS) in Escherichia coli. Molec. gen. Genet. 158, 149–155 (1977)
Lindahl, J., Jaskunas, S.R., Dennis, P.P., Nomura, M.: Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA and RNA polymerase subunits. Proc. nat. Acad. Sci. (Wash.) 72, 2743–2747 (1975)
Low, B.: Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J. Bact. 113, 798–812 (1975)
Miura, K.: Preparation of bacterial DNA by the phenol-pH 9-RNase method. In: Methods in enzymology (L. Grossman and K. Moldave, eds.), Vol. 12A, pp. 543–545. New York: Academic Press 1967
Nashimoto, H., Uchida, H.: Late steps in the assembly of 30S ribosomal proteins in Escherichia coli. J. molec. Biol. 96, 443–453 (1975)
O'Farrell, P.H.: High resolution two-dimensional electrophoresis of proteins. J. biol. Chem. 250, 4007–4021 (1975)
Saito, H., Uchida, H.: Initiation of the DNA replication of bacteriophage lambda in Escherichia coli K12. J. molec. Biol. 113, 1–25 (1977)
Saito, H., Uchida, H.: Organization and expression of the dnaJ and dnaK genes of Escherichia coli K12. Molec. gen. Genet. 164, 1–8 (1978)
Szybalski, E.H., Szybalski, W.: Physical mapping of the att-N region of coliphage lambda: apparent oversaturation of coding capacity in the gam-ral segment. Biochimie 56, 1497–1503 (1974)
Thomas, M., Cameron, J.R., Davis, R.W.: Viable molecular hybrids of bacteriophage lambda and eucaryotic DNA. Proc. nat. Acad. Sci. (Wash.) 71, 4579–4583 (1974)
Weigle, J.: Assembly of phage lambda in vitro. Proc. nat. Acad. Sci. (Wash.) 55, 1462–1466 (1966)
Yamamoto, M., Strycharz, W.A., Nomura, M.: Identification of genes for elongation factor Ts and ribosomal protein S2 in E. coli Cell 8, 129–138 (1976)
Yochem, J., Uchida, H., Sunshine, M., Saito, H., Georgopoulos, C.P., Feiss, M.: Genetic analysis of two genes, dnaJ and dnaK, necessary for Escherichia coli and bacteriophage lambda DNA replication. Molec. gen. Genet. 164, 9–14 (1978)
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Saito, H., Nakamura, Y. & Uchida, H. A transducing λ phage carrying grpE, a bacterial gene necessary for λ DNA replication, and two ribosomal protein genes, rpsP (S16) and rplS (L19). Molec. Gen. Genet. 165, 247–256 (1978). https://doi.org/10.1007/BF00332523
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DOI: https://doi.org/10.1007/BF00332523