Abstract
By means of renaturation kinetics of DNA of the three avian species Cairina domestica, Gallus domesticus and Columba livia domestica the following major DNA repetition classes were observed: a very fast reannealing fraction comprising about 15% of the DNA, a fast or intermediate reannealing fraction that makes up 10%, and a slow reannealing fraction of about 70%, which apparently renatures with single copy properties. — Comparing the reassociation behaviour of short (0.3 kb) and long (>2 kb) DNA fragments of duck and chicken it becomes apparent that only 12% (duck) and 28% (chicken) of the single copy DNA are interspersed with repetitive elements on 2 to 3 kb long fragments. The lengths of the repetitive sequences were estimated by optical hyperchromicity measurements, by agarose A-50 chromatography of S1 nuclease resistant duplexes and by electron microscopic measurements of the S1 nuclease resistant duplexes. It was found that in the case of the chicken DNA the single copy sequences alternating with middle repetitive ones are at least 2.3 kb long; the interspersed moderate repeats have a length average of at least 1.5 kb. The sequence length of the moderate repeats in duck DNA is smaller. The results show that the duck and the chicken genomes do not follow the short period interspersion pattern of genome organisation, characteristic of the eucaryotic organisms studied so far.
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References
Angerer, R.C., Davidson, E.H., Britten, R.J.: DNA sequence organization in the mollusc Aplysia California. Cell 6, 29–39 (1975)
Arthur, R.R., Straus, N.A.: DNA-sequence organization in the genome of the domestic chicken (Gallus domesticus). Canad. J. Biochem. 56, 257–263 (1978)
Atkin, N.B., Mattinson, G., Beçak, W., Ohno, S.: The comparative DNA content of 19 species of placental mammals, reptiles and birds. Chromosoma (Berl.) 17, 1–10 (1965)
Britten, R.J., Davidson, E.H.: Gene regulation for higher cells: A theory. Science 165, 349–357 (1969)
Britten, R.J., Davidson, E.H.: Repetitive and nonrepetitive DNA sequences and a speculation on the origins of evolutionary novelty. Quart. Rev. Biol. 46, 111–138 (1971)
Britten, R.J., Graham, D.E., Neufeld, B.R.: Analysis of repeating DNA sequences by reassociation. In: Methods in enzymology (L. Grossman and K. Moldave, eds.), vol. 29E, pp. 363–418. New York: Academic Press 1974
Cairns, J.: The chromosome of E. coli. Cold Spr. Harb. Symp. quant. Biol. 28, 43–46 (1963)
Crain, W.R., Davidson, E.H., Britten, R.J.: Contrasting patterns of DNA sequence arrangement in Apis mellifera (honeybee) and Musca domestica (housefly). Chromosoma (Berl.) 59, 1–12 (1976a)
Crain, W.R., Eden, F.C., Pearson, W.R., Davidson, E.H., Britten, R.J.: Absence of short period interspersion of repetitive sequences in the DNA of Drosophila melanogaster. Chromosoma (Berl.) 56, 309–326 (1976b)
Davidson, E.H., Galau, G.A., Angerer, R.C., Britten, R.J.: Comparative aspects of DNA organization in metazoa. Chromosoma (Berl.) 51, 253–259 (1975)
Davidson, E.H., Graham, D.E., Neufeld, B.R., Chamberlin, M.E., Amenson, C.S., Hough, B.R., Britten, R.J.: Arrangement and characterization of repetitive sequence elements in animal DNA. Cold Spr. Harb. Symp. quant. Biol. 38, 295–301 (1973b)
Davidson, E.H., Hough, R., Amenson, C.S., Britten, R.J.: General interspersion of repetitive with nonrepetitive sequence elements in the DNA of Xenopus. J. molec. Biol. 77, 1–23 (1973a)
Efstratiadis, A., Crain, W.R., Britten, R.J., Davidson, E.H., Kafatos, F.C.: DNA sequence organization in the lepidopteran Antheraea pernyi. Proc. nat. Acad. Sci. (Wash.) 73, 2289–2293 (1976)
Fasman, G.D.: Handbook of biochemistry and molecular Biology, 3rd ed., Nuclei acids-vol. II, pp. 300. Cleveland: CRC Press 1976
Goldberg, R.B., Crain, W.R., Ruderman, J.V., Moore, G.P., Barnett, T.R., Higgins, R.C., Gelfand, R.A., Galau, G.A., Britten, R.J., Davidson, E.H.: DNA sequence organization in the genomes of five marine invertebrates. Chromosoma (Berl.) 51, 225–251 (1975)
Graham, D.E., Neufeld, B.R., Davidson, E.H., Britten, R.J.: Interspersion of repetitive and non-repetitive DNA sequences in the sea urchin genome. Cell 1, 127–137 (1974)
Jimenez, E.S., Gonzales, J.L., Dominguez, J.C., Saloma, E.S.: Characterization of DNA from differentiated cells. Europ. J. Biochem. 45, 25–29 (1974)
Kirby, K.S.: Isolation of nuclei acids with phenolic solvents. In: Methods in enzymology (L. Grossman and K. Moldave, eds.), vol. 128, pp. 87–112. New York: Academic Press 1968
Kleinschmidt, A.K.: Monolayer techniques in electron microscopy of nucleic acid molecules. Methods in enzymology (L. Grossman and K. Moldave, eds.), vol. 128, pp. 361–377. New York: Academic Press 1968
Leipoldt, M.: Organisation und Aufbau des Vogelgenoms. Diplomarbeit, Univ. Freiburg, 1977
Mandel, M., Marmur, J.: Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. In: Methods in Enzymology (L. Grossman and K. Moldave, eds.), vol. 128, pp. 195–206. New York: Academic Press 1968
Manning, J.E., Schmid, C.W., Davidson, N.: Interspersion of repetitive and non-repetitive DNA sequences in the Drosophila melanogaster genome. Cell 4, 141–155 (1975)
McMaster, G.K., Carmichael, G.G.: Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridin orange. Proc. nat. Acad. Sci. (Wash.) 74, 4035–4038 (1977)
Noll, H.: Characterization of macromolecules by constant velocity sedimentation. Nature (Lond.) 215, 360–363 (1967)
Peacock, A.C., Dingman, C.W.: Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry 6, 1818–1827 (1967)
Rosen, J.M., Liarakos, C.D., O'Malley, B.W.: Effect of estrogen on gene expression in chick oviduct. I. Deoxyribonucleic acid-deoxyribonucleic acid renaturation studies. Biochemistry 12, 2803–2809 (1973)
Schmid, C.W., Deininger, P.L.: Sequence organization of the human genome. Cell 6, 345–358 (1975)
Sharp, P.A., Sugden, B., Sambrook, J.: Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis. Biochem. istry 12, 3055–3063 (1973)
Strom, C.M., Dorfman, A.: Distribution of 5-bromodeoxyuridine and thymidine in the DNA of developing chick cartilage. Proc. nat. Acad. Sci. (Wash.) 73, 1019–1023 (1976)
Strom, C.M., Dorfman, A.: Amplification of moderately repetitive DNA sequences during chick cartilage differentiation. Proc. nat. Acad. Sci. (Wash.) 73, 3428–3432 (1976b)
Studier, F.W.: Sedimentation studies of the size and shape of DNA. J. molec. Biol. 11, 373–390 (1965)
Wells, R., Royer, H.-D., Hollenberg, C.P.: Non Xenopus-like DNA sequence organization in the Chironomus tentans genome. Molec. gen. Genet. 147, 45–51 (1976)
Zimmermann, J.L., Goldberg, R.B.: DNA sequence organization in the genome of Nicotiana tabacum. Chromosoma (Berl.) 59, 227–252 (1977)
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Epplen, J.T., Leipoldt, M., Engel, W. et al. DNA sequence organisation in avian genomes. Chromosoma 69, 307–321 (1978). https://doi.org/10.1007/BF00332134
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DOI: https://doi.org/10.1007/BF00332134