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Die Lokalisation von Peroxydase und saurer Phosphatase in eosinophilen Leukocyten während der Reifung

Elektronenmikroskopisch-cytochemische Untersuchungen am Knochenmark von Ratte und Kaninchen

The localization of peroxidase and acid phosphatase in eosinophil leukocytes during maturation

Electron microscopical cytochemical studies on the bone marrow of the rat and rabbit

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Summary

The localization of peroxidase and acid phosphatase was investigated by electron microscopy and cytochemistry during the maturation of eosinophil leukocytes in the bone marrow of rats and rabbits. Peroxidase was demonstrated by the method of Graham and Karnovsky, acid phosphatase by a modified Gomori procedure. Promyelocytes contain homogeneous azurophil granules, myelocytes in addition crystalline eosinophil granules. Both granules are formed in condensing vacuoles, predominantly at the distal face of the Golgi apparatus. The azurophil granules are probably produced no longer from the myelocyte stage onwards, and are therefore largely eliminated by subsequent cell divisions. Peroxidase is present in all cisternae of the rough endoplasmic reticulum including the perinuclear cisterna, in smooth vesicles at the periphery of the Golgi complex, in all cisternae of the piled Golgi membranes, in condensing vacuoles and in all granules of promyelocytes and myelocytes; crystalline granules contain reaction product only in their matrix. From the metamyelocyte stage onwards, peroxidase disappears from the reticulum and Golgi complex. In mature eosinophils it is present only in granules. Acid phosphatase shows a similar distribution, but the reaction is rather variable and much weaker, especially in the reticulum. These results agree with the findings for the intracellular transport and segregation of secretory proteins obtained on other cell systems. In eosinophils, however, both condensing vacuoles and Golgi cisternae are functioning in the segregation of proteins.

Zusammenfassung

Die Lokalisation von Peroxydase und saurer Phosphatase während der Reifung eosinophiler Leukocyten im Knochenmark (Ratte, Kaninchen) wurde elektronenmikroskopisch und cytochemisch untersucht. Peroxydase wurde nach Graham and Karnovsky, saure Phosphatase nach Gomori nachgewiesen. Promyelocyten enthalten homogene, azurophile Granula, Myelocyten außerdem kristallhaltige, eosinophile Granula. Beide Granulaarten entstehen nacheinander in kondensierenden Vacuolen, vorwiegend an der distalen Fläche des Golgi-Apparates. Die azurophilen Granula werden wahrscheinlich vom Stadium des Myelocyten an nicht mehr gebildet und daher durch nachfolgende Zellteilungen weitgehend eliminiert. Peroxydase ist in allen Cisternen des rauhen endoplasmatischen Reticulums einschließlich der perinucleären Cisterne, in glattwandigen Bläschen an der Peripherie des Golgi-Komplexes, in allen Cisternen der Golgi-Membranstapel, in kondensierenden Vacuolen und in allen Granula der Promyelocyten und Myelocyten nachweisbar; die kristallhaltigen Granula zeigen Reaktionsprodukt ausschließlich in der Matrix. Vom Metamyelocyten-Stadium an verschwindet die Peroxydase aus Reticulum und Golgi-Apparat und ist in reifen Eosinophilen nur mehr in den Granula vorhanden. Saure Phosphatase zeigt eine gleichartige Verteilung, doch ist die Reaktion sehr variabel und bedeutend schwächer, besonders im Reticulum. Diese Ergebnisse stimmen mit den Befunden überein, die an anderen Zellsystemen für den intrazellulären Transport und die Segregation sekretorischer Proteine erhoben wurden. Bei Eosinophilen sind aber nicht nur kondensierende Vacuolen, sondern auch die Golgi-Cisternen an der Segregation von Proteinen beteiligt.

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  1. Elektronenmikroskopische Abteilung, Pathologisches Institut der Universität München, Deutschland

    F. Miller & V. Herzog

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Miller, F., Herzog, V. Die Lokalisation von Peroxydase und saurer Phosphatase in eosinophilen Leukocyten während der Reifung. Z. Zellforsch. 97, 84–110 (1969). https://doi.org/10.1007/BF00331873

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