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Genetic analysis of revertants for the nitrate reductase function of Nicotiana plumbaginifolia

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Summary

Spontaneous revertants of nitrate reductase (NR)-less mutants were isolated by screening for nitrate utilization in diploid NR− protoplast cultures of Nicotiana plumbaginifolia. The revertants contained in vivo NR activity in the case of apoenzyme mutants (nia) as well as of a cofactor-deficient (cnx) mutant. Revertants of the NIA type proved to be tetraploid, and genetic analysis showed that only one out of the four NR structural genes had reverted to a functional allele.

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Authors and Affiliations

  1. Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, Paardenstraat 65, B-1640, St. Genesius Rode, Belgium

    R. Dirks, I. Negrutiu, M. Heinderyckx & M. Jacobs

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  1. R. Dirks
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  2. I. Negrutiu
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  3. M. Heinderyckx
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  4. M. Jacobs
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Communicated by H. Böhme

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Dirks, R., Negrutiu, I., Heinderyckx, M. et al. Genetic analysis of revertants for the nitrate reductase function of Nicotiana plumbaginifolia . Mol Gen Genet 202, 309–311 (1986). https://doi.org/10.1007/BF00331656

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  • DOI: https://doi.org/10.1007/BF00331656

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