Summary
HgCl2-resistant strains of Acinetobacter sp. obtained from the soil at the Khaidarkan mercury mine (Kirghiz SSR) were found to contain, apart from large plasmids (∼60 kb), a small plasmid (∼7.5 kb) designated pKL1. It was established by conjugative crosses and transformation that pKL1 is a broad host range mobilizable plasmid and that it carries the Hgr determinant. The restriction map of pKL1 was constructed; the site of the Hgr determinant and the regions essential to replication were localized. A comparison of these results with earlier data suggests that microorganisms belonging to one microbiocenosis may carry Hgr determinants on plasmids with highly different structures and properties.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bagdasarian M, Timmis K (1982) Host: Vector systems for gene cloning in Pseudomonas. Current Topics Microbiol Immunol 98:47–67
Baumann P, Doudoroff M, Stanier R (1968) A study of the Moraxella group. II. Oxidative negative species (Genus Acinetobacter). J Bacteriol 95:1520–1541
Birnboim H, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucl Acids Res 7:1513–1523
Bukanov N, Fonshtein M, Tolmachev O, Yankovskii N, Fomichev Yu, Debabov V (1985) Erv. caratovora pectatlyase gene cloning. Genetika (USSR) 21, in press
Deneer HG, Slaney L, Maclean IW, Albritton WL (1982) Mobilization of nonconjugative antibiotic resista ce plasmids in Haemophilus ducreyi. J Bacteriol 149:726–732
Grinter N, Barth P (1976) Characterization of SmSu plasmids by restriction endonuclease cleavage and compatibility testing. J Bacteriol 128:394–400
Holmes D, Quigley M (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114:193–197
Khesin RB (1985) Mercury resistance R factors in bacteria isolated in the mercury-antimony deposit area. Mol Biol (USSR) 19:505–515
Khesin RB, Karasyova E (1984) Mercury-resistant plasmids in bacteria from a mercury and antimony deposit area. Mol Gen Genet 197:280–285
Kilbane JJ (1980) F factor mobilization of non-conjugative chimeric plasmids in Escherichia coli: general mechanisms and a role for site-specific recA-independent recombination at oriV1. J Mol Biol 143:73–93
Mandel M, Higa A (1970) Calcium dependent bacteriophage DNA infection. J Mol Biol 53:159–166
Maniatis T, Fritsch EE, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Messing J, Vieira J (1982) A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269–276
Miller JH (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Stalker D, Kolter R, Helinski D (1982) Plasmid R6K DNA replication. I. Complete nucleotide sequence of an autonomously replicating segment. J Mol Biol 161:33–43
Thomas C (1981) Molecular genetics of broad host range plasmid RK2. Plasmid, 5:10–19
Author information
Authors and Affiliations
Additional information
Communicated by D. Goldfarb
Deceased on July 16, 1985
Rights and permissions
About this article
Cite this article
Lomovskaya, O.L., Mindlin, S.Z., Gorlenko, Z.M. et al. A nonconjugative mobilizable broad host range plasmid of Acinetobacter sp. that determines HgCl2 resistance. Mol Gen Genet 202, 286–290 (1986). https://doi.org/10.1007/BF00331652
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331652