Summary
The kdsA gene of Escherichia coli was sequenced. It consists of 284 codons and is the last gene of a larger transcription unit. The mRNA is terminated by a rho-independent termination signal with the potential to be active in both orientations. This region is followed by another termination signal which seems to be active in the opposite orientation. Upstream of the kdsA gene a second open reading frame (ORF) was found; both this and the kdsA gene are transcribed in the same mRNA molecule when coded from the chromosome. In a plasmid-carried insert transcription starts from a cryptic promoter within the ORF preceding the kdsA gene. This promoter is not active in the intact chromosome.
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References
Berk AJ, Sharp PA (1977) Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12: 721–732
Brosius J, Dull TJ, Sleeter DD, Noller HF (1981) Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol 148:107–127
Favaloro J, Treisman R, Kamen R (1980) Transcription maps of polyoma virus-specific RNA: Analysis by two-dimensional nuclease S1 gel mapping. Methods Enzymol 65:718–749
Freudl R, Cole ST (1983) Cloning and molecular characterization of the ompA gene from Salmonella typhimurium. Eur J Biochem 134:497–502
Ikemura T (1981) Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: A proposal for a synonymous codon choice that is optimal for the E. coli translational system. J Mol Biol 151:389–409
Konigsberg W, Godson GN (1983) Evidence for use of rare codons in the dnaG gene and other regulatory genes of Escherichia coli. Proc Natl Acad Sci USA 80:687–691
Levin DH, Racker E (1959) Condensation of arabinose 5-phosphate and phosphorylenol pyruvate by 2-keto-3-deoxy-8-phosphooctonic acid synthetase. J Biol Chem 234:2532–2539
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, New York
Pirtle RM, Pirtle IL, Inouye M (1980) Messenger ribonucleic acid of the lipoprotein of the Escherichia coli outer membrane. J Biol Chem 255:199–209
Platt T, Bear DG (1983) Role of RNA polymerase, θ-factor, and ribosomes in transcription termination. In: Beckwith J, Davies J, Gallant JA (eds) Gene function in prokaryotes. Cold Spring Harbor Laboratory Press, New York, pp 123–161
Rick PD, Osborn MJ (1977) Lipid A mutants of Salmonella typhimurium. J Biol Chem 252:4895–4903
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Saraste M, Gay NJ, Eberle A, Runswick MJ, Walker JE (1981) The atp operon: nucleotide sequence of the genes for the γ, β and ε subunits of Escherichia coli ATP synthase. Nucleic Acids Res 9:5287–5296
Woisetschläger M, Högenauer G (1986) Cloning and characterization of the gene encoding 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli J Bacteriol 168:437–439
Young RA (1979) Transcription termination in the Escherichia coli ribosomal RNA operon rrnC. J Biol Chem 254:12725–12731
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Communicated by A. Böck
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Woisetschläger, M., Högenauer, G. The kdsA gene coding for 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase is part of an operon in Escherichia coli . Mol Gen Genet 207, 369–373 (1987). https://doi.org/10.1007/BF00331603
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DOI: https://doi.org/10.1007/BF00331603