Summary
The slow growing mutant cl1 of Paramecium, previously described (Sainsard, Claisse and Balmefrezol, 1974) differs from wild-type by a single recessive nuclear mutation and by a particular mitochondrial phenotype (Mcl) that gene cl 1 distinguishes from the wild-type mitochondrial phenotype (M+). A further analysis of these nucleo-mitochondrial interactions was carried out by confronting the genes cl 1 and cl +1 with mixed populations of M+ and Mcl mitochondria obtained after cytoplasmic exchange at conjugation. The following results were obtained: 1. M+ and Mcl mitochondria introduced respectively into mutant and wild-type cells do not multiply easily; 2. when a mixed population (M++Mcl) is established, both mitochondrial types are maintained during the growth of the F1 heterozygous cl 1/cl +1 clones; 3. when the nuclear segregation occurs in F2, the formation of homozygotes cl 1/cl 1 or cl +1 /cl +1 is soon followed by the segregation of the two mitochondrial types, Mcl or M+, reconstituting the two parental nucleo-mitochondrial associations.
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Communicated by W. Gajewski
This paper is dedicated to Professor T.M. Sonneborn on the occasion of his 70th birthday
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Sainsard-Chanet, A. Gene controlled selection of mitochondria in Paramecium . Molec. gen. Genet. 145, 23–30 (1976). https://doi.org/10.1007/BF00331553
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DOI: https://doi.org/10.1007/BF00331553