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Cyanelle DNA from Cyanophora paradoxa

Physical mapping and location of protein coding regions

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Summary

Cyanelles which have been found in few eukaryotic organisms are photosynthetically active organelles which strikingly resemble cyanobacteria. The complexity of the cyanelle genome in Cyanophora paradoxa (127 Kbp) is too low to consider them as independent organisms in a symbiotic relationship. In order to correlate cyanelle genome and gene structure with those of plastid chromosomes of other plants, a circular map of the cyanelle DNA from Cyanophora paradoxa (strain LB555 UTEX) has been constructed using the restriction endonucleases SalI (generating 6 DNA fragments), BamHI (6), SalI (5), XhoI (9), and BglII (19).

Besides the rRNA genes (16S, 23S, 5S), genes for 14 proteins have been located on this circular map. Among those are components of several multienzyme complexes involved in photosynthetic electron transport, as well as the large subunit of ribulose-1,5-bisphosphate carboxylase and two ribosomal proteins. All the probes used, were derived from a collection of spinach chloroplast DNA clones. Hybridization experiments showed signals to DNA fragments primarily from the large single-copy region of cyanelle DNA. The arrangement of genes on cyanelle DNA is different from that on spinach chloroplast DNA. However, genes which have been shown to be cotranscribed in spinach chloroplasts are also clustered on cyanelle DNA.

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Abbreviations

Kbp:

103 base pairs

Rubisco:

ribulose-1,5-bisphosphate carboxylase/oxygenase holoenzyme

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Authors and Affiliations

  1. Department of Biochemistry and Molecular, University of Arizona, 85721, Tucson, AZ, USA

    Hans J. Bohnert, Christine Michalowski & Sandra Bevacqua

  2. Department of Cellular Biology, University of Arizona, 85721, Tucson, AZ, USA

    Hans J. Bohnert, Christine Michalowski & Sandra Bevacqua

  3. Institut für Allgemeine Biochemie, Universität Wien, A-1090, Wien, Austria

    Hermann Mucke & Wolfgang Löffelhardt

  4. Ludwig-Boltzmann-Forschungsstelle für Biochemie, A-1090, Wien, Austria

    Hermann Mucke & Wolfgang Löffelhardt

Authors
  1. Hans J. Bohnert
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  2. Christine Michalowski
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  3. Sandra Bevacqua
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  4. Hermann Mucke
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  5. Wolfgang Löffelhardt
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Communicated by J. Schell

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Bohnert, H.J., Michalowski, C., Bevacqua, S. et al. Cyanelle DNA from Cyanophora paradoxa . Molec Gen Genet 201, 565–574 (1985). https://doi.org/10.1007/BF00331357

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  • DOI: https://doi.org/10.1007/BF00331357

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