Summary
Bacterial luciferase can be assayed rapidly and with high sensitivity both in vivo and in vitro. Here we demonstrate that the N-terminal hydrophobic domain of the α catalytic subunit of the luciferase enzyme is indispensable for enzyme activity, although N-terminal translational fusions with full luciferase activity can be obtained. Bacterial luciferase is therefore ideally suited as a reporter enzyme for gene fusion experiments. A list of vectors for the convenient use of the luciferase marker genes to monitor gene expression in vivo are presented.
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Olsson, O., Koncz, C. & Szalay, A.A. The use of the luxA gene of the bacterial luciferase operon as a reporter gene. Mol Gen Genet 215, 1–9 (1988). https://doi.org/10.1007/BF00331295
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DOI: https://doi.org/10.1007/BF00331295