Skip to main content
SpringerLink
Log in

A plasmid sequence from Rhizobium leguminosarum 300 contains homology to sequences near the octopine TL-DNA right border

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The DNA sequence from a Rhizobium leguminosarum 300 (RL300) plasmid that contains homology to the Tc-DNA of Agrobacterium tumefaciens is described. The RL300 sequence has 78% homology to a 359 bp sequence in the Tc-DNA of pTi15955. The RL300 homology starts approximately 100 bp from the 24 bp border sequence of the TL-DNA and ends approximately 3 bp from an IS66 homolog in the Tc-DNA. An unusual feature of the RL300 homology is the presence of 81 bp direct repeats with Tc-DNA homology, separated by 201 bp. One end of each direct repeat has a 12 bp palindrome. Four cloned sequences of RL300 with homology to the T DNA region were hybridized to plasmid lysates of RL300 derivatives to determine the source of each plasmid. The sequenced homolog, originally on pRH228, was isolated from pRL7JI; the other 3 homologs were isolated from the transmissable plasmids pRL7JI (pRH235) and pRL8JI (pRH235 and pRH236).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barker RF, Idler KB, Thompson DV, Kemp JD (1983) Nucleotide sequences of the T-DNA region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Mol Biol 2:335–350

    Google Scholar 

  • Better M, Lewis B, Corbin D, Ditta G, Helinski DR (1983) Structural relationships among Rhizobium meliloti symbiotic promoters. Cell 35:479–485

    Google Scholar 

  • Constantino P, Mauro ML, Micheli G, Risuelo G, Hooykaas PJJ, Schilperoort R (1981) Fingerprinting and sequence homology of plasmids from different virulent strains of Agrobacterium rhizogenes. Plasmid 5:170–182

    Google Scholar 

  • Donald RGK, Nees DW, Raymond CK, Loroch AI, Ludwig RA (1986) Characterization of three genomic loci encoding Rhizobium sp. strain ORS571 nitrogen fixation genes. J Bacteriol 165:72–81

    Google Scholar 

  • Engler G, Depicker A, Maenhaut R, Villarroel R, Van Montagu M, Schell J (1981) Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciens. J Mol Biol 152:183–208

    Google Scholar 

  • Gielen J, De Beuckeleer M, Seurinck, J, Deboeck F, De Greve H, Lemmers M, Van Montagu M, Schell J (1984) The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTIAch5. EMBO J 3:835–846

    Google Scholar 

  • Hadley RG, Szalay AA (1982) DNA sequences homologous to the T-DNA region of Agrobacterium tumefaciens are present in diverse Rhizobium species. Mol Gen Genet 188:361–369

    Google Scholar 

  • Heberlein, De Ley J, Tijtgat R (1967) Deoxyribonucleic acid homology and taxonomy of Agrobacterium, Rhizobium, and Chromobacterium. J Bacteriol 94:116–124

    Google Scholar 

  • Hepburn AC, White J (1984) Effect of right terminal repeat deletion on the oncogenicity of the T-DNA region of pTiT37. Plant Mol Biol 5:3–11

    Google Scholar 

  • Hirsch PR, Van Montagu M, Johnston AWB, Brewin NJ, Schell J (1980) Physical identification of bacteriocinogenic, nodulation and other plasmids in strains of Rhizobium leguminosarum. J Gen Microbiol 120:403–412

    Google Scholar 

  • Hooykaas PJJ, Peerbolte AJ, Regensburg-Tuink G, de Vries P, Schilperoort RA (1982) A chromosomal linkage map of Agrobacterium tumefaciens and a comparison with the maps of Rhizobium sp. Mol Gen Genet 188:12–17

    Google Scholar 

  • Huffman GA, White FF, Gordon MP, Nester EW (1984) Hairyroot-inducing plasmid: physical map and homology to tumor-inducing plasmids. J Bacteriol 157:269–276

    Google Scholar 

  • Johnston AWB, Hombrecher G, Brewin NJ, Cooper MC (1982) Two transmissible plasmids in Rhizobium leguminosarum strain 300. J Gen Microbiol 128:85–93

    Google Scholar 

  • Joos H, Timmerman B, Van Montagu M, Schell J (1983) Genetic analysis of transfer and stabilization of Agrobacterium DNA in plant cells. EMBO J 2:2151–2160

    Google Scholar 

  • Kaluza K, Hahn M, Hennecke H (1985) Repeated sequences similar to insertion elements clustered around the nif region of the Rhizobium japonicum genome. J Bacteriol 162:535–542

    Google Scholar 

  • Klee H, Montoya A, Horodyski FI, Lichtenstein C, Garfinkel D, Fuller S, Flores C, Peschon J, Nester EW, Gordon MP (1984) Nucleotide sequence of the tms genes of the pTiA6NC octopine Ti plasmid: Two gene products involved in plant tumorigenesis. Proc Natl Acad Sci USA 81:1728–1732

    Google Scholar 

  • Lemmers M, De Beuckeleer M, Holsters M, Zambryski P, Depicker A, Hernalsteens JP, Van Montagu M, Schell J (1980) Internal organization, boundaries and intergration of Ti-plasmid DNA in nopaline crown gall tumors. J Mol Biol 144:353–376

    Google Scholar 

  • Lichtenstein C, Klee H, Montoya A, Garfinkel D, Fuller S, Flores C, Nester E, Gordon M (1984) Nucleotide sequence and transcript mapping of the tmr gene of the pTiA6NC octopine T-plasmid: A bacteria involved in plant tumorigenesis. J Mol Appl Genet 2:354–362

    Google Scholar 

  • Machida Y, Sakurai M, Kiyokawa S, Ubasawa A, Suzuki Y, Ikeda J-E (1984) Nucleotide sequence of the insertion sequence found in the T-DNA region of mutant Ti plasmid pTiA66 and distribution of its homologues in octopine Ti-plasmid. Proc Natl Acad Sci USA 81:7495–7499

    Google Scholar 

  • Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560

    Google Scholar 

  • Peralta EG, Hellmiss R, Ream W (1986) Overdrive, a T-DNA transmission enhancer on the A. tumefaciens tumor-inducing plasmid. EMBO J 5:1137–1142

    Google Scholar 

  • Priefer UB, Burkardt HJ, Klipp W, Puhler A (1981) ISRl: An insertion element isolated from the soil bacterium Rhizobium lupini. Cold Spring Harbor Symp Quant Biol 45:87–91

    Google Scholar 

  • Quinto C, de la Vega H, Flores M, Leemans J, Cevallos MA, Pardo MA, Azpiroz R, Girard M de L, Calva E, Palacios R (1985) Nitrogenase reductase: a functional multigene family in Rhizobium phaseoli. Proc Natl Acad Sci USA 82:1170–1174

    Google Scholar 

  • Rosenberg C, Boistard P, Denarie J, Casse-Delbart F (1981) Genes controlling early and late functions in symbiosis are located on a mega-plasmid in Rhizobium meliloti. Mol Gen Genet 184:326–333

    Google Scholar 

  • Rosenberg M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–53

    Google Scholar 

  • Rostas K, Kondorosi E, Horvath B, Simoncsits A, Kondorosi A (1986) Conservation of extended promoter regions of nodulation genes in Rhizobium. Proc Natl Acad Sci USA 83:1757–1761

    Google Scholar 

  • Ruvkun GB, Long SR, Meade HM, van den Bos RC, Ausubel FM (1982) ISRml: a Rhizobium meliloti insertion sequence that transposes preferentially into nitrogen fixation genes. J Mol Appl Genet 1:405–418

    Google Scholar 

  • Sciaky D, Montoya AL, Chilton M-D (1978) Fingerprints of Agrobacterium Ti plasmids. Plasmid 1:238–253

    Google Scholar 

  • Scott KF, Rolfe BG, Schine J (1983) Biological nitrogen fixation: primary structure of the Rhizobium trifolii iron protein gene. DNA 2:149–155

    Google Scholar 

  • Shaw CH, Watson MD, Carter GH, Shaw CH (1984) The right hand copy of the nopaline Ti-plasmid 25 bp repeat is required for tumor formation. Nucleic Acids Res 12:6031–6041

    Google Scholar 

  • Shine J, Dalgarno L (1974) The 3′-terminal sequence of Escherichia coli 16 S ribosomal RNA: Complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71:1342–1346

    Google Scholar 

  • Shine J, Dalgarno L (1975) Determinant of cistron specificity in bacterial ribosomes. Nature 254:34–38

    Google Scholar 

  • Simpson RB, O'Hara PJ, Krook W, Montoya AL, Lichtenstein C, Gordon MP, Nester EW (1982) DNA from the A6S/2 crown gall tumors contains scrambled Ti-plasmid sequences near its junctions with the plant DNA. Cell 29:1009–1014

    Google Scholar 

  • Smith DR, Calvo JM (1980) Nucleotide sequence of the E. coli gene coding for dihydrofolate reductase. Nucleic Acids Res 8:2255–2275

    Google Scholar 

  • Tempe J, Petit A (1982) Opine utilization by Agrobacterium. In: Kahl G, Schell J (eds) Molecular Biology of Plant Tumors. Academic Press, New York, pp 427–449

    Google Scholar 

  • Wang K, Herrera-Estrella L, Van Montagu M, Zambryski P (1984) Right bp terminus sequence of the nopaline T-DNA is essential for and determines direction of DNA transfer from Agrobacterium to the plant genome. Cell 38:455–462

    Google Scholar 

  • White FF, Taylor BH, Huffman GA, Gordon, MP, Nester EW (1985) Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes. J Bacteriol 164:33–44

    Google Scholar 

  • Yadav NS, Vanderleyden J, Bennett DR, Barnes WM, Chilton M-D (1982) Short direct repeats flank the T-DNA on a nopaline Ti plasmid. Proc Natl Acad Sci USA 79:6322–6326

    Google Scholar 

  • Zambryski P, Depicker A, Kruger K, Goodman HM (1982) Tumor induction by Agrobacterium tumefaciens: analysis of the boundaries of T-DNA. J Mol Appl Genet 1:361–370

    Google Scholar 

Download references

Author information

Author notes

  1. Allen C. Yun

    Present address: Ricerca Inc, 44077, Painesville, OH, USA

  2. R. G. Hadley

    Present address: Plant Genetics Inc, 95616, Davis, CA, USA

Authors and Affiliations

  1. Boyce Thompson Institute for Plant Research, Cornell University, 14853, Ithaca, NY, USA

    Allen C. Yun, R. G. Hadley & Aladar A. Szalay

Authors
  1. Allen C. Yun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. G. Hadley
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aladar A. Szalay
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by J. Schell

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yun, A.C., Hadley, R.G. & Szalay, A.A. A plasmid sequence from Rhizobium leguminosarum 300 contains homology to sequences near the octopine TL-DNA right border. Mol Gen Genet 209, 580–584 (1987). https://doi.org/10.1007/BF00331166

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00331166

Key words

Search

Navigation