Summary
A temperature-sensitive mutant (dna-11) with the phenotype of a mutant defective in the initiation of DNA replication, was isolated from an Hfr-like FP2 donor of Pseudomonas aeruginosa. Reversion of its temperature-sensitive character was achieved by integrative suppression rather than by backmutation or an additional suppressor mutation. The dna-11 mutant proved to be helpful in stabilizing the Hfr status of the original host.
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Communicated by H. Böhme
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Herrmann, H., Klopotowski, T. & Günther, E. The Hfr status of Pseudomonas aeruginosa is stabilized by integrative suppression. Molec Gen Genet 204, 519–523 (1986). https://doi.org/10.1007/BF00331034
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DOI: https://doi.org/10.1007/BF00331034