Summary
The peptide antibiotic viomycin at a concentration of 10 μM inhibits E. coli ribosomes to the extent of about 70% as measured in the poly(U) system, and to about 85% in a natural mRNA (R17) system. Ribosomes from M. smegmatis show no activity at all at this concentration of the antibiotic. Experiments on the Mg2+ dependent dissociation and association of the ribosomal subunits revealed that viomycin stabilizes the 70S couples and promotes association of ribosomal subunits. This response is related to the drug action as indicated by the observation that viomycin resistant strains are not affected by viomycin with respect to dissociation and 70S couple information. A model for the inhibitory action of the drug is proposed.
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Cox, E.C., White, J.R., Flaks, J.G.: Streptomycin action and the ribosome. Proc. nat. Acad. Sci. (Wash.) 51, 703–709 (1964)
Davies, J.E.: Studies on the ribosomes of streptomycin-sensitive and resistant strains of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 51, 659–664 (1964)
Davies, J.E., Gorini, L., Davis, B.D.: Misreading of RNA codewords induced by aminoglycoside antibiotics. Molec. Pharmacol. 1, 93–106 (1965)
Dohme, F., Nierhaus, K.H.: Total reconstitution and assembly of 50S subunits from Escherichia coli ribosomes in vitro. J. molec. Biol. 107, 585–599 (1976)
Funatsu, G., Nierhaus, K., Wittmann, H.G.: Determination of allele types and amino acid exchanges in protein S12 of three streptomycin-resistant mutants of Escherichia coli. Biochim. biophys. Acta (Amst.) 287, 282–291 (1972)
Liou, Y.F., Tanaka, N.: Dual actions of viomycin on the ribosomal functions. Biochem. biophys. Res. Commun. 71, 477–483 (1976)
Masuda, K., Yamada, T.: Effect of viomycin on dihydrostreptomycin binding to bacterial ribosomes. Biochim. biophys. Acta (Amst.) 435, 333–339 (1976)
Mizugichi, Y., Suga, K., Masuda, K., Yamada, T.: Genetic and biochemical studies on drug-resistant mutants in Mycobacterium smegmatis. Jap. J. Microbiol. 18, 457–462 (1974)
Modolell, J., Vazquez, D.: The inhibition of ribosomal translocation by viomycin. Europ. J. Biochem. 81, 491–497 (1977)
Nierhaus, K.H., Montejo, V.: A protein involved in the peptidyltransferase activity of Escherichia coli ribosomes. Proc. nat. Acad. Sci. (Wash.) 70, 1931–1935 (1973)
Parmeggiani, A., Singer, C., Gottschalk, E.M.: Purification of the amino acid polymerisation factors from Escherichia coli. In: Methods in enzymology (Moldave, K., Grossman, L., eds.), Vol. XX, pp. 291–302. New York-London: Academic Press 1971
Suga, K., Mizugichi, Y.: Mapping of resistance markers in Mycobacterium smegmatis. Jap. J. Microbiol. 18, 139–147 (1974)
Tanaka, N., Igusa, S.: Effects of viomycin and polymyxin B on protein synthesis in vitro. J. Antibiot. (Tokyo) 21, 239–240 (1968)
Ulbrich, B., Nierhaus, K.H.: Pools of ribosomal proteins in Escherichia coli. Studies on the exchange of proteins between pools and ribosomes. Europ. J. Biochem. 57, 49–54 (1975)
Yamada, T., Masuda, K., Mizugichi, Y., Suga, K.: Altered ribosomes in antibiotic-resistant mutants of Mycobacterium smegmatis. Antimicrob. Agents Chemother. 9, 817–823 (1976)
Yamada, T., Masuda, K., Shoji, K., Hori, M.: Analysis of ribosomes from viomycin-sensitive and-resistant strains of Mycobacterium smegmatis. J. Bact. 112, 1–6 (1972)
Yamada, T., Masuda, K., Shoji, K., Hori, M.: Pleiotropic antibiotic resistance mutations associated with ribosomes and ribosomal subunits in Mycobacterium smegmatis. Antimicrob. Agents Chemother. 6, 46–53 (1974)
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Commumcated by E. Bautz
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Yamada, T., Nierhaus, K.H. Viomycin favours the formation of 70S ribosome couples. Molec. gen. Genet. 161, 261–265 (1978). https://doi.org/10.1007/BF00330999
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DOI: https://doi.org/10.1007/BF00330999