Summary
The nucleotide sequence of the ribosomal protein gene rpsO (S15) and its flanking region were determined. The amino acid sequence of S15 protein deduced from the nucleotide sequence is in good agreement with the published amino acid sequence with one exception. The nucleotide sequence shows two probable promoter sites about 100 nucleotides upstream from the initiation codon (AUG) of rpsO. Inspection of the sequence also revealed structural homology between the distal part of rpsO and the reported S15 binding region in 16S rRNA.
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References
Bachmann BJ (1983) Linkage map of Escherichia coli K-12. Microbiol Rev 47:180–230
Barry G, Squires C, Squires CL (1980) Attenuation and processing of RNA from the rplJL-rpoBC transcription unit of Escherichia coli. Proc Natl Acad Sci USA 77:3331–3335
Baughman G, Nomura M (1983) Localization of the target site for translational regulation of the L11 operon and direct evidence for translational coupling in Escherichia coli. Cell 34:979–988
Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW (1977) Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
Branlant C, Krol A, Machatt A, Ebel JP (1981) The secondary structure of the protein L1 binding region of ribosomal 23S RNA. Homologies with putative secondary structures of the L11 mRNA and of a region of mitochondrial 16S rRNA. Nucl Acids Res 9:293–307
Brosius J, Cate RL, Perlmutter AP (1982) Precise location of two promoters for the β-lactamase gene of pBR322. J Biol Chem 257:9205–9210
Brückner R, Matzura H (1981) In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/L12 in Escherichia coli. Mol Gen Genet 183:277–282
Burton FZ, Gross CA, Watanabe KK, Burgess RR (1983) The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12. Cell 32:335–349
Dean D, Yates JL, Nomura M (1981) Identification of ribosomal protein S7 as a repressor of translation within the str operon of E. coli. Cell 24:413–419
Fukuda R (1980) Autogenous regulation of the synthesis of ribosomal proteins, L10 and 17/12, in Escherichia coli. Mol Gen Genet 178:483–486
Hawley DK, McClure WR (1983) Compilation and analysis of Escherichia coli promoter DNA sequences. Nucl Acids Res 11:2237–2255
Kaltschmidt E, Wittmann HG (1970) Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for finger-printing of ribosomal proteins. Anal Biochem 36:401–412
Kitakawa M, Dabbs ER, Isono K (1979) Genes coding ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli. J Bacteriol 138:832–838
Lee JS, Gynheung A, Friesen JD, Isono K (1981) Cloning and the nucleotide sequence of the genes for Escherichia coli ribosomal proteins L28 (rpmB) and L33 (rpmG). Mol Gen Genet 184:218–223
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, New York
Maxmam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical clevages. In: Meth Enzymol 65:499–560
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York
Morinaga T, Funatsu G, Funatsu M, Wittmann HG (1976) Primary structure of the 16S rRNA binding protein S15 from Escherichia coli ribosomes. FEBS Lett 64:64:307–309
Nomura M, Held WA (1974) Reconstitution of ribosomes: Studies of ribosome structure, function and assembly. In: Nomura M, Tissières A, Lengyel P (eds) Ribosomes. Cold Spring Harbor Laboratory, New York, pp 193–223
Nomura M, Yates JL, Dean D, Post LE (1980) Feedback regulation of ribosomal protein gene expression in Escherichia coli: Structural homology of ribosomal RNA and ribosomal protein mRNA. Proc Natl Acad Sci USA 77:7084–7088
Olins PO, Nomura M (1981) Translational regulation by ribosomal protein S8 in Escherichia coli: Structural homology between rRNA binding site and feedback target on mRNA. Nucl Acids Res 9:1757–1764
Portier C (1982) Physical localisation and direction of transcription of the structural gene for Escherichia coli ribosomal protein S15. Gene 18:261–266
Post LE, Arfsten AE, Reusser F, Nomura M (1978) DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coli. Cell 15:215–229
Post LE, Strycharz GD, Nomura M, Lewis H, Dennis PP (1979) Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit in Escherichia coli. Proc Natl Acad Sci USA 76:1697–1701
Post LE, Arfsten AE, Davis GR, Nomura M (1980) DNA sequence of the promoter region for the α ribosomal protein operon in Escherichia coli. J Biol Chem 255:4653–4659
Pribnow D (1975) Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci USA 72:784–788
Rosenberg M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–353
Shine J, Dalgarno L (1974) The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: Complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71:1342–1346
Takata R (1978) Genetic studies of the ribosomal proteins in Escherichia coli XI. Mapping of the genes for L21, L27, S15 and S21 by using hybrid bacteria and overproduction of these proteins in the merodiploid strains. Mol Gen Genet 160:151–155
Takata R, Aoyagi M, Mukai T (1982) Cloning of rpsO, the gene for ribosomal protein S15 of Escherichia coli. Mol Gen Genet 188:334–337
Yates JL, Nomura M (1980) E. coli ribosomal protein L4 is a feedback regulatory protein. Cell 21:517–522
Yates JL, Arfsten AE, Nomura M (1980) In vitro expression of Escherichia coli ribosomal protein genes: Autogenous inhibition of translation. Proc Natl Acad Sci USA 77:1837–1841
Zimmermann RA (1979) Interactions among protein and RNA components of the ribosome. In: Chambliss G, Craven GR, Davies J, Davis K, Kahan L, Nomura M (eds) Ribosomes, structure, function and genetics. University Park Press, Baltimore, pp 135–169
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Takata, R., Mukai, T., Aoyagi, M. et al. Nucleotide sequence of the gene for Escherichia coli ribosomal protein S15 (rpsO). Mol Gen Genet 197, 225–229 (1984). https://doi.org/10.1007/BF00330967
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DOI: https://doi.org/10.1007/BF00330967