Summary
Fusions between protoplasts from haploid cytoplasmic atrazine resistant (CATR) and haploid cytoplasmic male sterile (CMS) Brassica napus plants were used to produce a diploid CMS/CATR cybrid. The hybrid nature of the cytoplasm was confirmed by comparing the EcoRI restriction fragment patterns of chloroplast and mitochondrial DNA from the cybrid with the parental patterns. The advantages of using haploid protoplasts for fusion experiments as well as the utilization of the CMS/CATR cybrid for hybrid seed production are discussed.
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Communicated by G. Melchers
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Chuong, P.V., Beyersdorf, W.D., Powell, A.D. et al. Somatic transfer of cytoplasmic traits in Brassica napus L. by haploid protoplast fusion. Mol Gen Genet 211, 197–201 (1988). https://doi.org/10.1007/BF00330594
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DOI: https://doi.org/10.1007/BF00330594