Summary
Streptomyces glaucescens GLA000 contains the integrated 15 kb DNA element pIJ408 which, during mating of the parent strain with S. lividans, can be transferred into recipient cells. In S. lividans cells, pIJ408 was found in an autonomously replicating form and in a chromosomally integrated state. In the majority of the S. lividans transconjugants studied, a deletion derivative pIJ408. 1 (12.4 kb) occurred. The deletion form was found in some strains only as a free plasmid, in others it was also chromosomally integrated. The integration region of pIJ408 was subcloned and precisely mapped by hybridization, restriction and sequencing analyses. The DNA junction fragments of the integrated plasmid in S. glaucescens, as well as the DNA fragment containing the attachment site of the S. lividans chromosome, were also cloned, submitted to detailed restriction analysis and sequenced. The attachment site of pIJ408 (attP) and the junctions of its integrated form with the chromosomal DNA in S. glaucescens (attL and attR) contain an identical 43 bp sequence. The chromosomal attachment site in S. lividans (attB) differs from the S. glaucescens att sequence by a single base substitution. The similarities between attachment sites of SLP1, pMEA100, pSAM2 and pIJ408 are discussed.
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References
Andrews BJ, Proteau GA, Beatty LG, Sadowski PD (1985) The FLP recombinase of the 2 μ circle DNA of yeast: interaction with its target sequences. Cell 40:795–803
Arnold W, Pühler A (1988) A family of high-copy-number plasmid vectors with single end-label sites for rapid nucleotide sequencing. Gene 70:171–179
Boccard F, Pernodet J-L, Friedmann A, Guerineau M (1988) Sitespecific integration of plasmid pSAM2 in Streptomyces lividans and S. ambofaciens. Mol Gen Genet 212:432–439
Boccard F, Smokvina T, Pernodet J-L, Friedmann A, Guérineau M (1989a) Structural analysis of loci involved in pSAM2 sitespecific integration in Streptomyces. Plasmid, in press
Boccard F, Smokvina T, Pernodet J-L, Friedmann A, Guérineau M (1989b) The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophages. EMBO J 8:973–980
Bolivar F, Backman K (1979) Plasmids of Escherichia coli as cloning vectors. Methods Enzymol 68:245–267
Botstein D, Kleckner N (1977) Translocation and illegitimate recombination by the tetracycline resistance element Tn10. In: Bukhari IA, Shapiro JA, Adhya SL (eds) DNA: insertion elements, plasmids, and episomes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 185–203
Brown DP, Chiang S-JD, Tuan JS, Katz L (1988) Site-specific integration in Saccharopolyspora erythrea and multisite integration in Streptomyces lividans of actinomycete plasmid pSE101. J Bacteriol 170:2287–2295
Chater KF, Bruton CJ, Foster SG, Tobek J (1985) Physical and genetic analysis of IS110, a transposable element of Streptomyces coelicolor A3(2). Mol Gen Genet 200:235–239
Chater KF, Henderson DJ, Bibb MJ, Hopwood DA (1988) Genome flux in Streptomyces coelicolor and other Streptomycetes and its possible relevance to the evolution of mobile antibiotic resistance determinants. In: Kingsman AJ, Chater KF, Kingsman SM (eds) Transposition. Cambridge University Press, Cambridge, pp 7–42
Chung S-T (1987) Tn4556, a 6.8-kilobase-pair transposable element of Streptomyces fradiae. J Bacteriol 169:4436–4441
Cohen A, Bar-Nir D, Goedeke ME, Parag Y (1985) The integrated and free states of Streptomyces griseus plasmid pSG1. Plasmid 13:41–50
Enquist L, Weisberg R (1977) Flexibility in attachment-site recognition by λ integrase. In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA: insertion elements, plasmids, and episomes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 343–348
Frischauf A-M, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170:827–842
Golden JW, Robinson SJ, Haselkorn R (1985) Rearrangement of nitrogen fixation genes during heterocyst differentiation in the cyanobacterium Anabaena. Nature 314:419–423
Hasegawa M, Hintermann G, Simonet J-M, Crameri R, Piret J, Hütter R (1985) Certain chromosomal regions in Streptomyces glaucescens tend to carry amplifications and deletions. Mol Gen Genet 200:375–384
Hintermann G, Crameri R, Kieser T, Hütter R (1981) Restriction analysis of the Streptomyces glaucescens genome by agarose gel electrophoresis. Arch Microbiol 130:218–222
Hoess RH, Abremski K (1984) Interaction of the bacteriophage P1 recombinase Cre with the recombining site loxP. Proc Natl Acad Sci USA 81:1026–1029
Hopwood DA, Hintermann G, Kieser T, Wright HM (1984) Integrated DNA sequences in three streptomycetes form related autonomous plasmids after transfer to Streptomyces lividans. Plasmid 11:1–16
Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM, Schrempf H (1985) Genetic manipulation of Streptomyces: a laboratory manual. The John Innes Foundation, Norwich
Hsu P-L, Ross W, Landy A (1980) The λ phage att site: functional limits and interaction with Int protein. Nature 285:85–91
Hütter R, Eckhardt T (1988) Genetic manipulation. In: Goodfellow M, Williams ST, Mordarski M (eds) Actinomyces in biotechnology, Academic Press, London, pp 89–184
Iida S, Hiestand-Nauer R (1986) Localized conversion at the crossover sequences in the site-specific DNA inversion system of bacteriophage P1. Cell 45:71–79
Iida S, Kulka I, Meyer J, Arber W (1987) Amplification of drug resistance genes flanked by inversely repeated IS1 elements: involvement of IS1-promoted DNA rearrangement before amplification. J Bacteriol 169:1447–1453
Kieser T (1984) Factors affecting the isolation of cccDNA from Streptomyces lividans and Escherichia coli. Plasmid 12:19–36
Kuhstoss S, Richardson MA, Nagaraja Rao R (1989) Site-specific integration in Streptomyces ambofaciens: localization of integration functions in S. ambofaciens plasmid pSAM2. J Bacteriol 171:16–23
Lee SC, Omer CA, Brasch MA, Cohen SN (1988) Analysis of recombination occurring at SLP1 att sites. J Bacteriol 170:5806–5813
Lydiate DJ, Ikeda H, Hopwood DA (1986) A 2.6 kb DNA sequence of Streptomyces coelicolor A3(2) which functions as a transposable element. Mol Gen Genet 203:79–88
Madoń J, Moretti P, Hütter R (1987) Site-specific integration and excision of pMEA100 in Nocardia mediterranei. Mol Gen Genet 209:257–264
Maniatis T, Frítsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Maxam AM, Gilbert W (1980) Sequencing end-labelled DNA with base specific chemical cleavages. Methods Enzymol 65:499–560
Messing J, Crea R, Seeburg PH (1981) A system for shotgun DNA sequencing. Nucleic Acids Res 9:309–321
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Mizuuchi K, Weisberg R, Enquist L, Mizuuchi M, Buraczynska M, Foeller C, Hsu P-L, Ross W, Landy A (1981) Structure and function of the phage λ att site: size, int-binding sites, and location of the crossover point. Cold Spring Harbor Symp Quant Biol 45:429–437
Moretti P, Hintermann G, Hütter R (1985) Isolation and characterization of an extrachromosomal element from Nocardia mediterranei. Plasmid 14:126–133
Murray NE, Brammar WJ, Murray K (1977) Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet 150:53–61
Norrander J, Kempe T, Messing J (1983) Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26:101–106
Omer CA, Cohen SN (1984) Plasmid formation in Streptomyces: excision and integration of the SLP1 replicon at a specific chromosomal site. Mol Gen Genet 196:429–438
Omer CA, Cohen SN (1986) Structural analysis of plasmid and chromosomal loci involved in site-specific excision and integration of the SLP1 element of Streptomyces coelicolor. J Bacteriol 166:999–1006
Omer CA, Stein D, Cohen SN (1988) Site-specific insertion of biologically functional adventitious genes into the Streptomyces lividans chromosome. J Bacteriol 170:2174–2184
Pernodet J-L, Simonet J-M, Guérineau M (1984) Plasmids in different strains of Streptomyces ambofaciens: free and integrated form of plasmid pSAM2. Mol Gen Genet 198:35–41
Reiter W-D, Palm P, Yeats S (1989) Transfer RNA genes frequently serve as integration sites for prokaryotic genetic elements. Nucleic Acids Res 17:1907–1914
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain termination inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Toussaint A, Faelen M, Bukhari AI (1977) Mu-mediated illegitimate recombination as an integral part of the Mu life cycle. In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA: insertion elements, plasmids, and episomes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 275–285
Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
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Sosio, M., Madon, J. & Hütter, R. Excision of pIJ408 from the chromosome of streptomyces glaucescens and its transfer into Streptomyces lividans . Mol Gen Genet 218, 169–176 (1989). https://doi.org/10.1007/BF00330580
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DOI: https://doi.org/10.1007/BF00330580