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The soybean chloroplast genome: Construction of a circular restriction site map and location of DNA regions encoding the genes for rRNAs, the large subunit of the ribulose-1,5-bisphosphate carboxylase and the 32 KD protein of the photosystem II reaction center

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Summary

Chloroplast DNA was isolated in preparative amounts from purified chloroplasts from soybean (Glycine max.). This DNA upon digestion with the restriction enzymes SalI, PstI, and SmaI yields 5, 9, and 12 fragments, respectively. The total genome size is between 150–152 kilobase pairs. A complete circular SalI-PstI-SmaI restriction site map has been constructed. The circular genome contains two inverted repeats of about 21–23 kbp separated by a single copy region of about 24 kbp. The rDNA region is part of the inverted repeat. The genes for the large subunit of the ribulose 1,5-bisphosphate carboxylase is localized about five kbp away from the gene coding for the 32 KD protein of the photosystem II reaction center. The close proximity of these two genes seems to be characteristic for chloroplast genomes of the legume family.

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Abbreviations

Kbp:

kilobase pairs

cpDNA:

chloroplast DNA

LSU:

large subunit

P32:

32 kilodalton thylakoid protein of the reaction center of photosystem II

EtBr:

ethidium bromide

SSC:

standard saline buffer (0.15 M NaCl, 0.015 M Na-citrate)

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Author notes

  1. William Ortiz

    Present address: Department of Plant and Soil Biology, University of California, 108, Hilgard Hall, 94720, Berkeley, CA, USA

Authors and Affiliations

  1. Laboratoire de Biochimie, Université de Neuchâtel, Ch. de Chantemerle 18, CH-2000, Neuchâtel, Switzerland

    Albert Spielmann, William Ortiz & Erhard Stutz

Authors
  1. Albert Spielmann
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  2. William Ortiz
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  3. Erhard Stutz
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Communicated by J. Schell

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Spielmann, A., Ortiz, W. & Stutz, E. The soybean chloroplast genome: Construction of a circular restriction site map and location of DNA regions encoding the genes for rRNAs, the large subunit of the ribulose-1,5-bisphosphate carboxylase and the 32 KD protein of the photosystem II reaction center. Mol Gen Genet 190, 5–12 (1983). https://doi.org/10.1007/BF00330317

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  • DOI: https://doi.org/10.1007/BF00330317

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