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Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli

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Summary

The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5′-phosphoryl and 3′-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690.

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Authors and Affiliations

  1. Department of Experimental Chemotherapy, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, 565, Suita, Osaka, Japan

    Yoshizumi Ishino, Hideo Shinagawa, Kozo Makino & Atsuo Nakata

  2. Department of Protein Structure, Institute for Protein Research, Osaka University, Yamadaoka, 565, Suita, Osaka, Japan

    Susumu Tsunasawa & Fumio Sakiyama

Authors
  1. Yoshizumi Ishino
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  2. Hideo Shinagawa
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  3. Kozo Makino
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  4. Susumu Tsunasawa
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  5. Fumio Sakiyama
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  6. Atsuo Nakata
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Additional information

Communicated by M. Takanami

On leave from Takara Shuzo Co., Ltd., Kyoto, Japan

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Ishino, Y., Shinagawa, H., Makino, K. et al. Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli . Molec Gen Genet 204, 1–7 (1986). https://doi.org/10.1007/BF00330179

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  • DOI: https://doi.org/10.1007/BF00330179

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