Summary
Nuclei were isolated from fixed liver tissue of diploid and triploid Rana pipiens and of rat. While the frog liver nuclei present a single ploidy class on the basis of Feulgen absorption measurements, rat liver contained diploid, tetraploid, and some octoploid nuclei. Nuclear areas within single ploidy classes varied over wide ranges, especially in the frog material. The mode of this variation was dependent on ploidy. Microspectrophotometric measurements of several protein components were compared to ploidy and nuclear volume. General protein methods indicated a linear relationship to nuclear volume. Protein-bound sulfhydryl and disulfide groups were not related to nuclear volume, but could be related to ploidy. Protein tyrosine values showed a partial dependence on both factors.
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This investigation was supported by a grant from the U.S. Public Health Service, GM-10003-03.
Post-Doctoral Trainee under U.S. Public Health Training Grant to the Department of Pathology, University of Florida, 5T1 GM 1142-03.
Supported by a Career Development award from the National Institute of Child Health and Human Development, K3-DH-6176-03.
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Bachmann, K., Cowden, R.R. Quantitative cytophotometric studies on polyploid liver cell nuclei of frog and rat. Chromosoma 17, 181–193 (1965). https://doi.org/10.1007/BF00330080
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DOI: https://doi.org/10.1007/BF00330080