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Intraplasmid recombination in Streptomyces lividans 66

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Summary

An Escherichia coli-Streptomyces shuttle plasmid pIF132 containing two direct mel repeats was constructed. While pIF132 replicated relatively stably in E. coli (Rec+ or recA), its structure was unstable in S. lividans: recombination between the mel repeats resulted in a smaller plasmid, pIF138. Furthermore, pIF132 formed oligomers extensively in E. coli but not in S. lividans.

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Author notes

  1. Jane F. -Y. Tsai

    Present address: The Rockefeller University, 1230 York Ave., Box 32, New York, USA

  2. Shuang-en Chuang

    Present address: Laboratory of Cellular and Molecular Biology, University of Wisconsin, 53706, Madison, WI, USA

Authors and Affiliations

  1. Institute of Microbiology and Immunology, National Yangming Medical College, Taipei, Taiwan, Republic of China

    Carton W. Chen, Jane F. -Y. Tsai & Shuang-en Chuang

  2. Panlabs Fermtech Division, Panlabs Taiwan, Ltd., PO Box 26-127, Taipei, Taiwan, Republic of China

    Carton W. Chen & Jane F. -Y. Tsai

Authors
  1. Carton W. Chen
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  2. Jane F. -Y. Tsai
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  3. Shuang-en Chuang
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Communicated by N.D.F. Grindley

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Chen, C.W., Tsai, J.F.Y. & Chuang, Se. Intraplasmid recombination in Streptomyces lividans 66. Mol Gen Genet 209, 154–158 (1987). https://doi.org/10.1007/BF00329851

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  • DOI: https://doi.org/10.1007/BF00329851

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