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Elektronenmikroskopische Untersuchungen über den Sekretionsmodus des Parathormones

Beitrag zu einer lysosomalen Mitbeteiligung bei Sekretionsvorgängen in endokrinen Drüsen

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Summary

After EDTA-stimulation the blood calcium level is instantaneously lowered for a restricted time. EDTA- (8%) injected Wistar rats of 150–200 g body weight were killed in various intervals after the injections and the ultrastructural changes of the parathyroid glands were examined.

In the beginning only changes in the so-called “osmiophilic bodies” are observed. The electron dense contents of these granules become flocculent and vesiculated. Later they gain close relation to lipid bodies. A confluence of the two bodies seems likely. In the final phase the vesicular Golgi field and the rough endoplasmic reticulum expand markedly, indicating an increased activity. Thus a phase of release and one of restitution can be distinguished. The participation of lysosomes in the secretion of the parathormone is discussed.

Zusammenfassung

Durch EDTA-Injektion wird der Blutcalciumspiegel akut und befristet gesenkt. 150–200 g schwere Wistarratten erhalten je 2 ml EDTA (8%) i.p. und werden in verschiedenen Zeitpunkten nach der Injektion getötet. Die Veränderungen der Ultrastruktur der Epithelkörperchen werden untersucht.

Zu Versuchsbeginn lassen sich lediglich Veränderungen an den osmiophilen Körpern nachweisen, die bevorzugt am Kapillarpol der Zelle liegen. Diese Einschlüsse zeigen eine Auflockerung und oft eine bläschenartige Umwandlung ihrer elektronendichten Innenstruktur. Sie treten später in enge räumliche Beziehungen zu den Fettkörpern. In der Spätphase läßt sich eine starke Entfaltung der vesikulär umgebildeten Golgizentren und des rauhen endoplasmatischen Retikulum beobachten. Somit kann eine Ausschüttungs- und eine Restitutionsphase unterschieden werden. Eine Beteiligung der Lysosomen an der Parathormonsekretion wird diskutiert.

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Authors and Affiliations

  1. Ludwig Aschoff-Haus, Pathologisches Institut der Universität Freiburg i. Br., Deutschland

    Hanspeter Rohr & Brigitte Krässig

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  1. Hanspeter Rohr
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  2. Brigitte Krässig
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Ausgeführt mit Unterstützung durch die Deutsche Forschungsgemeinschaft.

Wesentliche Teile der vorliegenden Arbeit werden von Brigitte Krässig der Medizinischen Fakultät der Universität Freiburg i. Br. als Inauguraldissertation vorgelegt.

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Rohr, H., Krässig, B. Elektronenmikroskopische Untersuchungen über den Sekretionsmodus des Parathormones. Zeitschrift für Zellforschung 85, 271–290 (1968). https://doi.org/10.1007/BF00328842

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  • DOI: https://doi.org/10.1007/BF00328842

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