Abstract
A modified BrdU-Hoechst-Giemsa technique permitted the demonstration of easily reproducible replication patterns in the somatic chromosomes of Amphibia. These banding patterns allow for the first time a precise identification of all chromosomes and the analysis of the patterns of replication in the various stages of S-phase in Amphibia. Several possibilities for the use of this technique were demonstrated on three frog species of the family Ranidae, all differing greatly in their DNA-content. With this method, the homomorphic chromosome pair No. 4 in Rana esculenta could be identified as sex-specific chromosomes of the XX/XY-type. All male animals exhibit an extremely late replicating region in the Y-chromosome, which is lacking in the X-chromosome; in the female animals, both X-chromosomes replicate synchronously. These sex-specific chromosomes cannot be distinguished by other banding techniques. In the highly heteromorphic ZZ/ZW-sex chromosome system of Pyxicephalus adspersus a synchronous replication of the two Z-chromosomes of male animals and a very late replication of the short arm of the W-chromosome of female animals was demonstrated. These results support the assumption that there is no dosage compensation for Z-linked or X-linked genes by the sex chromosome inactivation mechanism in the sex chromosomes of Amphibia.
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References
Allen, J.W., Latt, S.A.: Analysis of sister chromatid exchange formation in vivo in mouse spermatogonia as a new test system for environmental mutagens. Nature (Lond.) 260, 449–451 (1976a)
Allen, J.W., Latt, S.A.: In vivo BrdU-33258 Hoechst analysis of DNA replication kinetics and sister chromatid exchange formation in mouse somatic and meiotic cells. Chromosoma (Berl.) 58, 325–340 (1976b)
Berger, L.: Morphology of the F1 generation of various crosses within Rana esculenta complex. Acta Zool. Cracov 3, 301–324 (1968)
Bianchi, N.O., Bianchi, M.S., Vidal-Rioja, L.: Heterochromatin late replication and secondary constrictions in the chromosome complement of Leptodactylus ocellatus. Caryologia (Firenze) 26, 397–403 (1973)
Bianchi, N.O., Molina, J.O.: DNA replication patterns in somatic chromosomes of Leptodactylus ocellatus (Amphibia, Anura). Chromosoma (Berl.) 22, 391–400 (1967)
Biedermann, B.M., Florence, D., Lin, C.C.: Cytogenetic analysis of great horned owls (Bubo virginianus). Cytogenet. Cell Genet. 28, 79–86 (1980)
Giorgi, F., Galleni, L.: The lampbrush chromosomes of Rana esculenta L. (Amphibia, Anura). Caryologia (Firenze) 25, 107–123 (1972)
King, M.: C-banding studies on Australian hylid frogs: secondary constriction structure and the concept of euchromatin transformation. Chromosoma (Berl.) 80, 191–217 (1980)
Latt, S.A.: Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc. nat. Acad. Sci. (Wash.) 70, 3395–3399 (1973)
Macgregor, H.C., Horner, H.: Heteromorphism for chromosome 1, a requirement for normal development in crested newts. Chromosoma (Berl.) 76, 111–122 (1980)
Morescalchi, A.: Amphibia. In: Cytotaxonomy and vertebrate evolution (A.B. Chiarelli and E. Capanna, eds.), pp. 233–348. London-New York: Academic Press 1973
Morescalchi, A., Filosa, S.: Osservazioni sui cromosomi piumosi di Rana esculenta L. Atti. Soc. Peloritana Sc. fis. mat. nat. 11, 211–219 (1965)
Ohno, S.: Sex chromosomes and sex-linked genes. Berlin-Heidelberg-New York: Springer 1967
Ragghianti, M., Bucci-Innocenti, S., Mancino, G.: Bandeggiatura indotta da “C-, G-e Q-staining methods” e pattern di replicazione dei cromosomi di Trituras. Rend. Acc. Naz. Lincei, Ser. VIII, 55, 764–770 (1973)
Ruiz, I.R.G., Soma, M., Beçak, W.: Nucleolar organizer regions and constitutive heterochromatin in polyploid species of the genus Odontophrynus (Amphibia, Anura). Cytogenet. Cell Genet. 29, 84–98 (1981)
Schmid, M.: Chromosome banding in Amphibia. I. Constitutive heterochromatin and nucleolus organizer regions in Bufo and Hyla. Chromosoma (Berl.) 66, 361–388 (1978a)
Schmid, M.: Chromosome banding in Amphibia. II. Constitutive heterochromatin and nucleolus organizer regions in Ranidae, Microhylidae and Rhacophoridae. Chromosoma (Berl.) 68, 131–148 (1978b)
Schmid, M.: Chromosome banding in Amphibia. IV. Differentiation of GC- and AT-rich chromosome regions in Anura. Chromosoma (Berl.) 77, 83–103 (1980a)
Schmid, M.: Chromosome banding in Amphibia. V. Highly differentiated ZW/ZZ sex chromosomes and exceptional genome size in Pyxicephalus adspersus (Anura, Ranidae). Chromosoma (Berl.) 80, 69–96 (1980b)
Schmid, M., Olert, J., Klett, C.: Chromosome banding in Amphibia. III. Sex chromosomes in Triturus. Chromosoma (Berl.) 71, 29–55 (1979)
Schmid, W.: DNA replication patterns of the heterochromosomes in Gallus domesticus. Cytogenet. Cell Genet. 1, 344–352 (1962)
Singh, L., Purdom, I.F., Jones, K.W.: Satellite DNA and evolution of sex chromosomes. Chromosoma (Berl.) 59, 43–62 (1976)
Singh, L., Purdom, I.F., Jones, K.W.: Human chromosomes contain repeated sequences related to sex chromosome associated DNA in snakes, birds, and insects. Cytogenet. Cell Genet. 25, 204 (1979)
Tunner, H.G.: Das Albumin und andere Bluteiweiße bei Rana ridibunda Pallas, Rana lessonae Camerano, Rana esculenta Linné und deren Hybriden. Z. Zool. Syst. Evol. Forsch. 11, 219–233 (1973)
Tunner, H.G.: Die klonale Struktur einer Wasserfroschpopulation. Z. Zool. Syst. Evol. Forsch. 12, 309–314 (1974)
Ullerich, F.-H.: Weitere Untersuchungen über Chromosomenverhältnisse und DNS-Gehalt bei Anuren (Amphibia). Chromosoma (Berl.) 21, 345–368 (1967)
Uzzell, T., Günther, R., Berger, L.: Rana ridibunda and Rana esculenta: a leaky hybridogenetic system (Amphibia, Salientia). Proc. Acad. Nat. Sci. (Philadelphia) 128, 147–171 (1977)
Uzzell, T., Hotz, H., Berger, L.: Genome exclusion in gametogenesis by an interspecific Rana hybrid: evidence from electrophoresis of individual oocytes. J. exp. Zool. 214, 251–259 (1980)
Vogel, W., Bauknecht, T.: Differential chromatid staining by in vivo treatment as a mutagenicity test system. Nature (Lond.) 260, 448–449 (1976)
Wimber, D.E., Prensky, W.: Autoradiography with meiotic chromosomes of the male newt (Triturus viridescens) using H3-thymidine. Genetics 48, 1731–1738 (1963)
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Schempp, W., Schmid, M. Chromosome banding in amphibia. Chromosoma 83, 697–710 (1981). https://doi.org/10.1007/BF00328528
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DOI: https://doi.org/10.1007/BF00328528