Summary
To elucidate possible biochemical pathways of the formation of halogenated C1/C2-hydrocarbons we investigated the product pattern of the reaction of chloroperoxidase (CPO) with natural substances including the haloform reaction as a second step. The analysis of the reaction products was performed by HRGC-ECD. We report here first results. As substrates we used acetic acid and acetone while KBr and NaCl served as halogen sources. The system acetic acid/KBr/CPO formed tribromomethane as the main product, with dibromochloromethane (CHBr2Cl) and 1,1,2,2-tetrachloroethane as by-products. Incubation with acetone resulted in the formation of CHBr3 as the main product, with CHBr2Cl, CH2Br-CH2Br, CHCl2-CHCl2, CHCl2-CCl3 and trichloroethene as side products, while the reaction of acetone with NaCl and CPO yielded chloroform as a main product.
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Walter, B., Ballschmiter, K. Elucidation of biochemical formation of C1/C2-(bromo-/chloro)-hydrocarbons. Fresenius J Anal Chem 341, 564–566 (1991). https://doi.org/10.1007/BF00328497
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DOI: https://doi.org/10.1007/BF00328497