Skip to main content
Log in

Movement of Drosophila melanogaster transposable elements other than P elements in a P-M hybrid dysgenic cross

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The Drosophila melanogaster mobile DNA sequences P factors and P elements transpose at elevated rates when P strain males are mated to M strain females in a hybrid dysgenic cross (Engels 1983). Isofemale lines derived from such a cross were analysed by in situ hybridisation using cloned copies of the transposable elements copia, 412 and F. It was found that lines derived from dysgenic crosses showed a statistically significant number of new sites for these elements when compared to a non-dysgenic control cross. This result suggests a functional coupling of copia, 412 and F transposition and some component present in the P-M dysgenic system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bridges CB (1935) Salivary chromosome maps. J Hered 26:60–64

    Google Scholar 

  • DiNocera PP, Digan ME, Dawid IB (1983) A family of oligoadenylate-terminated transposable sequences in Drosophila melanogaster. J Mol Biol 168:715–727

    Google Scholar 

  • Engels WR (1983) The P family of transposable elements in Drosophia. A Rev Genet 17:315–344

    Google Scholar 

  • Feinberg AP, Vogelstein B (1984) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    Google Scholar 

  • Flavell AJ, Ish-Horowicz D (1983) The origin of extrachromosomal circular copia elements. Cell 34:415–419

    Google Scholar 

  • Flavell AJ (1984) Role of reverse transcription in the generation of extrachromosomal copia mobile genetic elements. Nature 310:514–516

    Google Scholar 

  • Gerasimova TI, Mizrokhi LJ, Georgiev GP (1984) Transposition bursts in genetically unstable Drosohpila melanogaster. Nature 309:714–716

    Google Scholar 

  • Gerasimova TI, Matjunina LV, Mizrokhi LJ, Georgiev GP (1985) Successive transposition explosions in Drosophila melanogaster and reverse transpositions of mobile dispersed genetic elements. EMBO J 4:3773–3779

    Google Scholar 

  • Karess RE, Rubin GM (1984) Analysis of P transposable element functions in Drosophila. Cell 38:135–146

    Google Scholar 

  • Kidwell MG, Kidwell JF, Sved JA (1977) Hybrid dysgenesis in Drosophila melanogaster: a syndrome of aberrant traits including mutation, sterility and male recombination. Genetics 86:813–833

    Google Scholar 

  • Kidwell MG, Novy JB (1979) Hybrid dysgenesis in Drosophila melanogaster: sterility resulting from gonadal dysgenesis in the P-M system. Genetics 92:1127–1140

    Google Scholar 

  • Kidwell MG (1981) Hybrid dysgenesis in Drosophila melanogaster: the genetics of cytotype determination in a neutral strain. Genetics 98:275–290

    Google Scholar 

  • Rubin GM, Brorein WJ, Dunsmuir P, Flavell AJ, Levis R, Strobel E, Toole JJ, Young E (1981) Copia-like transposable elements in the Drosophila genome. Cold Spring Harb Symp Quant Biol 45:619–628

    Google Scholar 

  • Rubin GM, Kidwell MG, Bingham PM (1982) The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell 29:987–994

    Google Scholar 

  • Shrimpton AE, Montgomery EA, Langley CH (1986) Om mutations in Drosophila ananassae are linked to insertions of a transposable element. Genetics 114:125–135

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by D.J. Finnegan

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lewis, A.P., Brookfield, J.F.Y. Movement of Drosophila melanogaster transposable elements other than P elements in a P-M hybrid dysgenic cross. Mol Gen Genet 208, 506–510 (1987). https://doi.org/10.1007/BF00328147

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00328147

Key words

Navigation