Abstract
Two Clostridium thermocellum strains were improved for ethanol tolerance, to 5% (v/v), by gradual adaptation and mutation. The best mutant gave an ethanol yield of 0.37 g/g substrate, with a growth yield 1.5 times more than its parent. Accumulation of acids and reducing sugars by the mutant strain with 5% (v/v) ethanol was lower than that of the parent strain with 1.5% (v/v) ethanol.
Similar content being viewed by others
References
Barker, S.B. & Summerson, W.H. 1941 The colorimetric determination of lactic acid in biological material. Journal of Biological Chemistry 138, 535–554.
Bender, J., Vatcharapijarn, Y. & Jeffries, T.W. 1985 Characteristics and adaptability of some new isolates of Clostridium thermocellum. Applied and Environmental Microbiology 49, 475–477.
Freier, D., Mothershed, C.P. & Wiegel, J. 1988 Characterization of Clostridium thermocellum JW 20. Applied and Environmental Microbiology 54, 204–211.
Herrero, A.A. & Gomez, R.F. 1980 Development of ethanol tolerance in Clostridium thermocellum. Effect of growth temperature. Applied and Environmental Microbiology 40, 571–577.
Leeper, S.A. 1992 Membrane separations in the recovery of biofuels and biochemicals. An update review. In Separation and Purification Technology, eds Li, N.N. & Colo, J.M. pp. 99–194. New York: Marcel Dekker.
Lovitt, R.W., Kim, B.H., Shen, G.J. & Zeikus, J.G. 1988 Solvent production by microorganisms. CRC Critical Reviews in Biotechnology 7, 107–186.
Lynd, L.R. 1989 Production of ethanol from lignocellulosic materials using thermophilic bacteria. Critical evaluation of potential and review. Advances in Biochemical Engineering and Biotechnology 38, 2–52.
Mori, Y. 1990 Characterization of a symbiotic coculture of Clostridium thermohydrosulfuricum YM3 and Clostridium thermocellum YM4. Applied and Environmental Microbiology 56, 37–42.
Sai Ram, M., Rao, C.V. & Seenayya, G. 1991a Characteristics of Clostridium thermocellum strain SS8: a broad saccharolytic thermophile. World Journal of Microbiology and Biotechnology 7, 272–275.
Sai Ram, M. & Seenayya, G. 1989 Ethanol production by Clostridium thermocellum SS8—a newly isolated thermophilic bacterium. Biotechnology Letters 11, 589–592.
Sai Ram, M. & Seenayya, G. 1991 Production of ethanol from straw and bamboo pulp by primary isolates of Clostridium thermocellum. World Journal of Microbiology and Biotechnology 7, 372–378.
Sai Ram, M., Swamy, M.V. & Seenayya, G. 1991b Fermentation characteristics of ethanol producing isolates of Clostridium thermocellum. Indian Journal of Microbiology 31, 175–180.
Sato, K., Goto, S., Yonemura, S., Sekine, K., Okuma, E., Takagi, Y., Hon-Nami, K. & Saiki, T. 1992 Effect of yeast extract and Vitamin B12 on ethanol production from cellulose by Clostridium thermocellum I-1-B. Applied and Environmental Microbiology 58, 734–736.
Slapack, G.E., Russell, I. & Stewart, G.G. 1985 Thermophilic Bacteria and Thermotolerant Yeasts for Ethanol Production. NRCC No. 24410 project report. Ottawa: Division of Energy, National Research Council of Canada.
Tailliez, P., Girard, H., Longin, R., Beguin, P. & Millet, T. 1989a Cellulose fermentation by an asporogenous mutant and an ethanol tolerant mutant of Clostridium thermocellum. Applied and Environmental Microbiology 55, 203–206.
Tailliez, P., Girard, H., Millet, J. & Beguin, P. 1989b. Enhanced cellulose fermentation by an asporogenous and ethanol tolerant mutant of Clostridium thermocellum. Applied and Environmental Microbiology 55, 207–211.
Weimer, P.J. & Zeikus, J.G. 1977 Fermentation of cellulose and cellobiose by Clostridium thermocellum in the absence and presence of Methanobacterium thermo-autotrophicum. Applied and Environmental Microbiology 33, 289–297.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rani, K.S., Swamy, M.V., Sunitha, D. et al. Improved ethanol tolerance and production in strains of Clostridium thermocellum . World J Microbiol Biotechnol 12, 57–60 (1996). https://doi.org/10.1007/BF00327802
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00327802