Skip to main content
Log in

Improved ethanol tolerance and production in strains of Clostridium thermocellum

  • Research
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Two Clostridium thermocellum strains were improved for ethanol tolerance, to 5% (v/v), by gradual adaptation and mutation. The best mutant gave an ethanol yield of 0.37 g/g substrate, with a growth yield 1.5 times more than its parent. Accumulation of acids and reducing sugars by the mutant strain with 5% (v/v) ethanol was lower than that of the parent strain with 1.5% (v/v) ethanol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barker, S.B. & Summerson, W.H. 1941 The colorimetric determination of lactic acid in biological material. Journal of Biological Chemistry 138, 535–554.

    Google Scholar 

  • Bender, J., Vatcharapijarn, Y. & Jeffries, T.W. 1985 Characteristics and adaptability of some new isolates of Clostridium thermocellum. Applied and Environmental Microbiology 49, 475–477.

    Google Scholar 

  • Freier, D., Mothershed, C.P. & Wiegel, J. 1988 Characterization of Clostridium thermocellum JW 20. Applied and Environmental Microbiology 54, 204–211.

    Google Scholar 

  • Herrero, A.A. & Gomez, R.F. 1980 Development of ethanol tolerance in Clostridium thermocellum. Effect of growth temperature. Applied and Environmental Microbiology 40, 571–577.

    Google Scholar 

  • Leeper, S.A. 1992 Membrane separations in the recovery of biofuels and biochemicals. An update review. In Separation and Purification Technology, eds Li, N.N. & Colo, J.M. pp. 99–194. New York: Marcel Dekker.

    Google Scholar 

  • Lovitt, R.W., Kim, B.H., Shen, G.J. & Zeikus, J.G. 1988 Solvent production by microorganisms. CRC Critical Reviews in Biotechnology 7, 107–186.

    Google Scholar 

  • Lynd, L.R. 1989 Production of ethanol from lignocellulosic materials using thermophilic bacteria. Critical evaluation of potential and review. Advances in Biochemical Engineering and Biotechnology 38, 2–52.

    Google Scholar 

  • Mori, Y. 1990 Characterization of a symbiotic coculture of Clostridium thermohydrosulfuricum YM3 and Clostridium thermocellum YM4. Applied and Environmental Microbiology 56, 37–42.

    Google Scholar 

  • Sai Ram, M., Rao, C.V. & Seenayya, G. 1991a Characteristics of Clostridium thermocellum strain SS8: a broad saccharolytic thermophile. World Journal of Microbiology and Biotechnology 7, 272–275.

    Google Scholar 

  • Sai Ram, M. & Seenayya, G. 1989 Ethanol production by Clostridium thermocellum SS8—a newly isolated thermophilic bacterium. Biotechnology Letters 11, 589–592.

    Google Scholar 

  • Sai Ram, M. & Seenayya, G. 1991 Production of ethanol from straw and bamboo pulp by primary isolates of Clostridium thermocellum. World Journal of Microbiology and Biotechnology 7, 372–378.

    Google Scholar 

  • Sai Ram, M., Swamy, M.V. & Seenayya, G. 1991b Fermentation characteristics of ethanol producing isolates of Clostridium thermocellum. Indian Journal of Microbiology 31, 175–180.

    Google Scholar 

  • Sato, K., Goto, S., Yonemura, S., Sekine, K., Okuma, E., Takagi, Y., Hon-Nami, K. & Saiki, T. 1992 Effect of yeast extract and Vitamin B12 on ethanol production from cellulose by Clostridium thermocellum I-1-B. Applied and Environmental Microbiology 58, 734–736.

    Google Scholar 

  • Slapack, G.E., Russell, I. & Stewart, G.G. 1985 Thermophilic Bacteria and Thermotolerant Yeasts for Ethanol Production. NRCC No. 24410 project report. Ottawa: Division of Energy, National Research Council of Canada.

    Google Scholar 

  • Tailliez, P., Girard, H., Longin, R., Beguin, P. & Millet, T. 1989a Cellulose fermentation by an asporogenous mutant and an ethanol tolerant mutant of Clostridium thermocellum. Applied and Environmental Microbiology 55, 203–206.

    Google Scholar 

  • Tailliez, P., Girard, H., Millet, J. & Beguin, P. 1989b. Enhanced cellulose fermentation by an asporogenous and ethanol tolerant mutant of Clostridium thermocellum. Applied and Environmental Microbiology 55, 207–211.

    Google Scholar 

  • Weimer, P.J. & Zeikus, J.G. 1977 Fermentation of cellulose and cellobiose by Clostridium thermocellum in the absence and presence of Methanobacterium thermo-autotrophicum. Applied and Environmental Microbiology 33, 289–297.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rani, K.S., Swamy, M.V., Sunitha, D. et al. Improved ethanol tolerance and production in strains of Clostridium thermocellum . World J Microbiol Biotechnol 12, 57–60 (1996). https://doi.org/10.1007/BF00327802

Download citation

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00327802

Key words

Navigation