Skip to main content
Log in

Determination of glucose-fructose oxidoreductase activity in whole cells of Zymomonas mobilis

  • Research
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

A method for the determination of glucose-fructose oxidoreductase (GFOR) activity in whole cells of Zymomonas mobilis is described. The K m and the theoretical V max for GFOR were 192 g glucose.l-1 and 17 g gluconic acid.g-1 cell.h-1, respectively. The changes in enthalpy (31.1 kJ.mol-1), entropy (0.41 kJ.K-1), and Gibbs free energy (-97.5 kJ.mol-1) related to glucose to gluconic acid conversion were also determined.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Aldrich, H.C., McDowell, L., Barbosa, M.D.S., Yomano, L.P., Scopes, R.K. & Ingram, L.O. 1992 Immunocytochemical localization of glycolytic and fermentative enzymes in Zymomonas mobilis. Journal of Bacteriology 174, 4504–4508.

    Google Scholar 

  • Barrow, K.D., Leigh, D.A., Rogers, P.L. & Warr, R.G. 1984 Sorbitol production of Zymomonas mobilis. Applied Microbiology and Biotechnology 20, 225–232.

    Google Scholar 

  • Hurdman, M.J. & Scopes, R.K. 1988 The kinetics of glucose-fructose oxidoreductase from Zymomonas mobilis. European Journal of Biochemistry 173, 203–209.

    Google Scholar 

  • Loos, H., Krämer, R., Sahm, H. & Sprenger, G.A. 1994 Sorbitol promotes growth of Zymomonas mobilis in environments with high concentrations of sugar: evidence for a physiological function of glucose-fructose oxidoreductase in osmoprotection. Journal of Bacteriology 174, 7688–7693.

    Google Scholar 

  • Möllering, H. & Bergmeyer, H.U. 1984 Methods of Enzymatic Analysis, Vol. 6, 3rd edn, pp. 220–227. Basel: Verlag Chemie.

    Google Scholar 

  • Moore, W.J. 1985 Physical Chemistry. London: Longman.

    Google Scholar 

  • Owusu, R.K. & Makhzoum, A. 1992 Heat inactivation of lipase from psychotrophic Pseudomonas fluorescens P38: activation parameters and enzyme stability at low or ultra-high temperatures. Food Chemistry 44: 261–268.

    Google Scholar 

  • Paterson, S.L., Fane, A.G., Fell, C.J.D., Chun, U.H. & Rogers, P.L. 1988 Sorbitol and gluconate production in a hollow fibre membrane reactor by immobilized Zymomonas mobilis. Biocatalysis 1, 217–229.

    Google Scholar 

  • Zachariou, M. & Scopes, R.K. 1986. Glucose-fructose oxidoreductase, a new enzyme isolated from Zymomonas mobilis that is responsible for sorbitol production. Journal of Bacteriology 167, 863–869.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Erzinger, G.S., Silveira, M.M., Vitolo, M. et al. Determination of glucose-fructose oxidoreductase activity in whole cells of Zymomonas mobilis . World J Microbiol Biotechnol 12, 22–24 (1996). https://doi.org/10.1007/BF00327793

Download citation

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00327793

Key words

Navigation