Abstract
A thermophilic fungus, Humicola grisea var thermoidea, produced in liquid culture two endoxylanases (1,4-β-d-xylan-xylanohydrolase, EC 3.2.1.8) with M r of 95 (Xyl I) and 13 (Xyl II) kDa. PAGE of the crude culture filtrate and of each fraction obtained by gel filtration produced three and one band, respectively. Cross-reaction of the culture filtrate and each fraction with polyclonal antibodies prepared against Xyl II produced two and one precipitin bands, respectively. Hydrolysis of wheat straw and rice husk xylan was maximal using a combination of Xyl I and Xyl II. The products formed after hydrolysis, xylo-oligosaccharides and traces of xylose, indicated an endotype enzyme action and the co-operative activities of the xylanases.
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Anand, L., Krishnamurthy, S. & Vithayathil, P.J. 1990 Purification and properties of xylanase from the thermophilic fungus, Humicola lanuginosa (Griffon and Maublanc) Bunce. Archives of Biochemistry and Biophysics 276 546–553.
Biely, P., Mislovicova, D. & Toman, R. 1988 Ramazol brilliant blue-xylan: a soluble chromogenic substrate for xylanases. Methods in Enzymology 160, 536–538.
Chaudhary, S., Thakur, I.S., Goel, R. & Johri, B.N. 1988 Purification and characterization of two thermostable xylanases from Melanocarpus albomyces. Biochemistry International 17, 563–575.
Davis, B.J. 1964 Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121, 404–427.
Frederick, M.M., Kiang, C.H., Frederick, J.R. & Reilly, P. J. 1985 Purification and characterization of endo-xylanases from Aspergillus niger. I. Two isozymes active on xylan backbones near branch points. Biotechnology and Bioengineering 27, 525–532.
Gorbacheva, I.V. & Rodionova, N.A., 1977 Studies on xylan degrading enzymes II. Action pattern of endo-1,4-β-xylanase from Aspergillus niger str. 14 on xylan and xylooligosaccharides. Biochimica et Biophysica Acta 484, 94–102.
Hashimoto, S., Muramatsu, T. & Funatsu, M. 1971 Studies on xylanase from Trichoderma viride. Part I. Isolation and some properties of crystalline xylanase. Agricultural and Biological Chemistry 35, 501–508.
Jain, M.K., Kapoor, K.K. & Mishra, M.M. 1979 Cellulase activity, degradation of cellulose and lignin, and humus formation by thermophilic fungi. Transactions of the British Mycological Society 73, 85–89.
Koyama, H., Ujiie, M., Taniguchi, H. & Sasaki, T. 1990 Purification and some properties of xylan-hydrolysing enzymes from Robillarda sp. y-20. Enzyme and Microbial Technology 12, 218–224.
Michael, P. 1984 Ecological Methods for Field and Laboratory Investigations. New Delhi: Tata McGraw-Hill.
Morales, P., Madarro, A., Perez-Gozales, J.A., Sendra, J.M., Pinaga, F. & Flors, A. 1993 Purification and characterization of alkaline xylanases from Bacillus polymyxa. Applied and Environmental Microbiology 59, 1376–1382.
Ouchterlony, O. 1958. Diffusion-gel methods of immunological analysis. Progress in Allergy 5, 1–78.
Thakur, I.S. 1989 Fractionation and analysis of allergenicity of allergens from Prosopis juliflora pollen. International Archives of Allergy and Applied Immunology 90, 124–129.
Thakur, I.S., Rana, B.K. & Johri, B.N. 1991 Multiplicity of xylanases in Humicola grisea var thermoidea. In Xylan and Xylanases, ed Visser, J. pp. 511–514. North Holland: Elsevier.
Trevelyn, W.E., Procter, P.D. & Harrison, J.S. 1950 Detection of sugars on paper chromatogram. Nature 166, 444–445.
Updegraff, D.M. 1969. Semi-microdetermination of cellulose in biological materials. Analytical Biochemistry 32, 420–424.
Whitaker, J.R. 1963 Determination of molecular weight of proteins by gel filtration on Sephadex. Analytical Chemistry 35, 1950–1957.
Wong, K.K.Y., Tan, L.U.L. & Saddler, J.N. 1986 Functional interactions among three xylanases from Trichoderma harzianum. Enzyme and Microbial Technology 8, 617–622.
Wong, K.K.Y., Tan, L.U.L. & Saddler, J.N. 1988 Multiplicity of β-1,4-xylanase in microorganisms: function and application. Microbiological Review 52, 305–317.
Yoshioka, H., Nagato, N., Chavanich, S., Nilubol, N. & Hayashida, S. 1981 Purification and properties of thermostable xylanase from Talaromyces bassochlamydoides XY-50. Agriculture and Biological Chemistry 45, 2425–2432.
Yu, E.K.C., Tan, L.U.L., Chen, M.K.H., Deschatelets, C.L. & Saddler, J.N. 1987 Production of thermostable xylanase by a thermophilic fungus, Thermoascus aurantiacus. Enzyme and Microbial Technology 9, 16–24.
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Rana, B.K., Johri, B.N. & Thakur, I.S. Formation and activities of xylanhydrolysing enzymes of Humicola grisea var thermoidea . World J Microbiol Biotechnol 12, 12–15 (1996). https://doi.org/10.1007/BF00327791
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DOI: https://doi.org/10.1007/BF00327791