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Genetic complementation of Agrobacterium tumefaciens Ti plasmid mutants in the virulence region

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Summary

Mutants with Tn5 insertions in the vir region of the Agrobacterium tumefaciens TiC58 plasmid are unable to form crown-gall tumors. Complementation tests of these vir region mutants were carried out by constructing merodiploids in a recombination-deficient strain. Each merodiploid possessed a mutant TiC58 plasmid and a recombinant plasmid containing either the homologous wild-type DNA region or the homologous region containing a second Tn5 insertion. The analysis identified six complementation groups. Mutations in one of these complementation groups were not complemented in trans and represent a cis-dominant locus. The mutation in one complementation group showed variation in host range.

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Authors and Affiliations

  1. Department of Plant Pathology, University of California, 95616, Davis, CA, USA

    Ronald C. Lundquist, Timothy J. Close & Clarence I. Kado

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  1. Ronald C. Lundquist
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  2. Timothy J. Close
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  3. Clarence I. Kado
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Communicated by M.M. Green

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Lundquist, R.C., Close, T.J. & Kado, C.I. Genetic complementation of Agrobacterium tumefaciens Ti plasmid mutants in the virulence region. Molec. Gen. Genet. 193, 1–7 (1984). https://doi.org/10.1007/BF00327406

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  • DOI: https://doi.org/10.1007/BF00327406

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